Document Detail

Modulation of retinoic acid-induced differentiation of human leukemia (HL-60) cells by serum factors and sphinganine.
MedLine Citation:
PMID:  2153047     Owner:  NLM     Status:  MEDLINE    
The human cell line HL-60 was used to investigate the role of protein kinase C in the regulation of retinoic acid-induced maturation of promyelocytic leukemia cells by growth and differentiation factors found in serum. Cells grown in serum-containing medium differentiated less than cells in serum-free medium due to several factors, including albumin binding of retinoic acid. Addition of an inhibitor (sphinganine) of protein kinase C, an enzyme that participates in cellular responses to many serum factors, facilitated the retinoic acid-induced differentiation. Cells treated with both retinoic acid and sphinganine produced more superoxide when stimulated by formylmethionylleucylphenylalanine; hence, this combination generated a more functional population of cells. The ability of sphinganine to promote retinoic acid-induced differentiation suggests that retinoic acid therapy might be improved by the concurrent use of a modulator of protein kinase C activity.
V L Stevens; N E Owens; E F Winton; J M Kinkade; A H Merrill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  50     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-02-13     Completed Date:  1990-02-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  222-6     Citation Subset:  IM    
Department of Biochemistry, Emory University, Atlanta, Georgia 30322.
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MeSH Terms
Blood Physiological Phenomena*
Cell Differentiation / drug effects
Leukemia, Promyelocytic, Acute / metabolism,  pathology*
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Protein Kinase C / physiology
Sphingosine / analogs & derivatives*,  pharmacology
Superoxides / metabolism
Tetradecanoylphorbol Acetate / pharmacology
Tretinoin / metabolism,  pharmacology*,  therapeutic use
Grant Support
Reg. No./Substance:
11062-77-4/Superoxides; 123-78-4/Sphingosine; 16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 764-22-7/safingol; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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