| Modulation of phagocytosis of apoptotic neutrophils by supernatant from dexamethasone-treated macrophages and annexin-derived peptide Ac(2-26). | |
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MedLine Citation:
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PMID: 15749912 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phagocytic clearance of apoptotic leukocytes plays an important role in the resolution of inflammation. The glucocorticoid-inducible protein annexin 1 and annexin 1-derived peptides show potent anti-inflammatory responses in acute and chronic inflammation. In this study, we report that the annexin 1-derived peptide (Ac(2-26)) significantly stimulates nonphlogistic phagocytosis of apoptotic polymorphonuclear leukocytes (PMNs) by human monocyte-derived macrophages (Mphi). Peptide Ac(2-26)-stimulated phagocytosis is accompanied by rearrangement of the Mphi actin cytoskeleton. To investigate the potential role of endogenous annexin on clearance of apoptotic cells, Mphi were cultured for 5 days in the presence of dexamethasone. Supernatants collected from dexamethasone-treated Mphi significantly enhanced the ability of naive Mphi to engulf apoptotic PMNs. This effect was blocked by an annexin blocking Ab, by immunodepletion of the supernatants, and by the formyl peptide receptor/lipoxin receptor antagonist Boc1. In addition, we show that bone marrow-derived Mphi from annexin 1-null mice present a 40% decreased phagocytosis of apoptotic PMNs compared with cells taken from littermate controls. In conclusion, these results emphasize the pivotal role of annexin 1 as mediator for clearance of apoptotic cells and expand its potential therapeutic role in controlling inflammatory diseases. |
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Authors:
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Paola Maderna; Simon Yona; Mauro Perretti; Catherine Godson |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 174 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-07 Completed Date: 2005-04-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 3727-33 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine and Therapeutics, The Conway Institute of Biomolecular and Biomedical Research, The Dublin Molecular Medicine Centre, University College Dublin, Dublin, Ireland. paola.maderna@ucd.ie |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Annexin A1 / deficiency, genetics, immunology, pharmacology* Apoptosis Cyclic AMP / metabolism Cyclic AMP-Dependent Protein Kinases / metabolism Dexamethasone / pharmacology Humans Interleukin-8 / biosynthesis Macrophages / drug effects*, immunology*, metabolism Mice Mice, Knockout Neutrophils / cytology*, immunology* Peptides Phagocytosis / drug effects Transforming Growth Factor beta / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Annexin A1; 0/Interleukin-8; 0/Peptides; 0/Transforming Growth Factor beta; 0/annexin A1 peptide (2-26); 50-02-2/Dexamethasone; 60-92-4/Cyclic AMP; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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