Document Detail


Modulation of peritoneal macrophage activity by the saturation state of the fatty acid moiety of phosphatidylcholine.
MedLine Citation:
PMID:  19466285     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
To determine the effects of saturated and unsaturated fatty acids in phosphatidylcholine (PC) on macrophage activity, peritoneal lavage cells were cultured in the presence of phosphatidylcholine rich in saturated or unsaturated fatty acids (sat PC and unsat PC, respectively), both used at concentrations of 32 and 64 microM. The treatment of peritoneal macrophages with 64 microM unsat PC increased the production of hydrogen peroxide by 48.3% compared to control (148.3 +/- 16.3 vs 100.0 +/- 1.8%, N = 15), and both doses of unsat PC increased adhesion capacity by nearly 50%. Moreover, 64 microM unsat PC decreased neutral red uptake by lysosomes by 32.5% compared to the untreated group (67.5 +/- 6.8 vs 100.0 +/- 5.5%, N = 15), while both 32 and 64 microM unsat PC decreased the production of lipopolysaccharide-elicited nitric oxide by 30.4% (13.5 +/- 2.6 vs 19.4 +/- 2.5 microM) and 46.4% (10.4 +/- 3.1 vs 19.4 +/- 2.5 microM), respectively. Unsat PC did not affect anion production in non-stimulated cells or phagocytosis of unopsonized zymosan particles. A different result pattern was obtained for macrophages treated with sat PC. Phorbol 12-miristate 13-acetate-elicited superoxide production and neutral red uptake were decreased by nearly 25% by 32 and 64 microM sat PC, respectively. Sat PC did not affect nitric oxide or hydrogen peroxide production, adhesion capacity or zymosan phagocytosis. Thus, PC modifies macrophage activity, but this effect depends on cell activation state, fatty acid saturation and esterification to PC molecule and PC concentration. Taken together, these results indicate that the fatty acid moiety of PC modulates macrophage activity and, consequently, is likely to affect immune system regulation in vivo.
Authors:
F C C Grando; C A Felício; A Twardowschy; F M Paula; V G Batista; L C Fernandes; R Curi; A Nishiyama
Related Documents :
2250025 - Increases in phosphatidic acid levels accompany sphingosine-stimulated proliferation of...
2827755 - Spin-label electron spin resonance study of bacteriophage m13 coat protein incorporatio...
6501425 - Shape changes in human erythrocytes induced by replacement of the native phosphatidylch...
8394975 - Dynamics of iron-ascorbate-induced lipid peroxidation in charged and uncharged phosphol...
16143465 - Influence of growth conditions on pseudomonas fluorescens strains: a link between metab...
8197705 - Effects of acetamide on experimentally-induced palicourea marcgravii (st hill) poisonin...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-22
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  42     ISSN:  1414-431X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  599-605     Citation Subset:  IM    
Affiliation:
Departamento de Fisiologia, Universidade Federal do Paraná, 81531-990 Curitiba, PR, Brasil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of blood neoangiogenesis and lymphatic vascularization in colorectal adenomas from patien...
Next Document:  Continuous spinal anesthesia versus combined spinal epidural block for major orthopedic surgery: pro...