Document Detail


Modulation of peristalsis by cannabinoid CB(1) ligands in the isolated guinea-pig ileum.
MedLine Citation:
PMID:  10696099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of cannabinoid drugs on peristalsis in the guinea-pig ileum was studied. Peristalsis was induced by delivering fluid into the oral end of an isolated intestinal segment. Longitudinal muscle reflex contraction, threshold pressure and threshold volume to trigger peristalsis, compliance of the intestinal wall during the preparatory phase (a reflection of the resistance of the wall to distension) and maximal ejection pressure during the emptying phase of peristalsis were measured. The cannabinoid agonists WIN 55,212-2 (0.3 - 300 nM) and CP55,940 (0.3 - 300 nM) significantly decreased longitudinal muscle reflex contraction, compliance and maximal ejection pressure, while increased threshold pressure and volume to elicit peristalsis. These effects were not modified by the opioid antagonist naloxone (1 microM) and by the alpha-adrenoceptor antagonist phentolamine (1 microM). The inhibitory effect of both WIN 55,212-2 and CP55,940 on intestinal peristalsis was antagonized by the cannabinoid CB(1) receptor antagonist SR141716A (0.1 microM), but not by the cannabinoid CB(2) receptor antagonist SR144528 (0.1 microM). In absence of other drugs, the CB(1) receptor antagonists SR141716A (0.01 - 1 microM) and AM281 (0.01 - 1 microM) slightly (approximatively 20%) but significantly increased maximal ejection pressure during the empty phase of peristalsis without modifying longitudinal muscle reflex contraction, threshold pressure, threshold volume to trigger peristalsis and compliance. It is concluded that activation of CB(1) receptors reduces peristalsis efficiency in the isolated guinea-pig, and that the emptying phase of peristalsis could be tonically inhibited by the endogenous cannabinoid system.
Authors:
A A Izzo; N Mascolo; M Tonini; F Capasso
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  129     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-04     Completed Date:  2000-05-04     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  984-90     Citation Subset:  IM    
Affiliation:
Department of Experimental Pharmacology, University of Naples 'Federico II', via D. Montesano 49, 80131 Naples, Italy. aaizzo@unina.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzoxazines
Bornanes / pharmacology
Cannabinoids / antagonists & inhibitors,  pharmacology
Compliance / drug effects
Cyclohexanols / pharmacology
Guinea Pigs
Ileum / drug effects*
Ligands
Male
Morpholines / pharmacology
Muscle Contraction / drug effects
Naphthalenes / pharmacology
Peristalsis / drug effects*
Piperidines / pharmacology
Pyrazoles / pharmacology
Receptors, Cannabinoid
Receptors, Drug / agonists,  antagonists & inhibitors,  drug effects*
Chemical
Reg. No./Substance:
0/AM 281; 0/Benzoxazines; 0/Bornanes; 0/Cannabinoids; 0/Cyclohexanols; 0/Ligands; 0/Morpholines; 0/Naphthalenes; 0/Piperidines; 0/Pyrazoles; 0/Receptors, Cannabinoid; 0/Receptors, Drug; 0/SR 144528; 134959-51-6/Win 55212-2; 158681-13-1/rimonabant; 83003-12-7/3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol
Comments/Corrections

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