Document Detail

Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of Cephamandole from Cephalosporin C.
MedLine Citation:
PMID:  11549439     Owner:  NLM     Status:  MEDLINE    
The modulation of penicillin G acylase (PGA) properties via immobilization techniques has been performed studying the acylation of 7-aminocephalosporanic acid with R-mandelic acid methyl ester. PGA from Escherichia coli, immobilized onto agarose activated with glycidol (glyoxyl-agarose), has been used for the design of a novel one-pot synthesis of Cephamandole in aqueous medium and without isolation of intermediates, through three consecutive biotransformations catalyzed by D-amino acid oxidase, glutaryl acylase and PGA.
M Terreni; G Pagani; D Ubiali; R Fernández-Lafuente; C Mateo; J M Guisán
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  11     ISSN:  0960-894X     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-10     Completed Date:  2002-03-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  2429-32     Citation Subset:  IM    
Pharmaceutical Biocatalysis Laboratories, Dipartimento di Chimica Farmaceutica, Università degli Studi di Pavia, via Taramelli 12, 27100, Pavia, Italy.
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MeSH Terms
Biochemistry / methods*
Cefamandole / chemical synthesis*,  metabolism
Cephalosporins / chemistry*,  metabolism
Enzymes, Immobilized / chemistry*,  metabolism
Escherichia coli / enzymology
Penicillin Amidase / chemistry*,  metabolism*
Sepharose / chemistry
Reg. No./Substance:
0/Cephalosporins; 0/Enzymes, Immobilized; 34444-01-4/Cefamandole; 61-24-5/cephalosporin C; 9012-36-6/Sepharose; EC Amidase

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