Document Detail


Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis.
MedLine Citation:
PMID:  21692747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
TIR (Toll/interleukin-1 receptor) domain-containing proteins play a crucial role in innate immunity in eukaryotes. Brucella is a highly infectious intracellular bacterium that encodes a TIR domain protein (TcpB) to subvert host innate immune responses to establish a beneficial niche for pathogenesis. TcpB inhibits NF-κB (nuclear factor κB) activation and pro-inflammatory cytokine secretions mediated by TLR (Toll-like receptor) 2 and TLR4. In the present study, we have demonstrated that TcpB modulates microtubule dynamics by acting as a stabilization factor. TcpB increased the rate of nucleation as well as the polymerization phases of microtubule formation in a similar manner to paclitaxel. TcpB could efficiently inhibit nocodazole- or cold-induced microtubule disassembly. Microtubule stabilization by TcpB is attributed to the BB-loop region of the TIR domain, and a point mutation affected the microtubule stabilization as well as the TLR-suppression properties of TcpB.
Authors:
Girish K Radhakrishnan; Jerome S Harms; Gary A Splitter
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  439     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-15     Completed Date:  2011-11-14     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  79-83     Citation Subset:  IM    
Affiliation:
Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Brucella melitensis / genetics,  metabolism*
Microtubules / drug effects,  metabolism*
Nocodazole / pharmacology
Protein Structure, Tertiary
Receptors, Interleukin-1 / genetics,  metabolism*
Toll-Like Receptor 2 / genetics,  metabolism
Toll-Like Receptor 4 / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
1R01AI073558/AI/NIAID NIH HHS; R01 AI073558/AI/NIAID NIH HHS; R21 AI088038/AI/NIAID NIH HHS; R21 AI088038/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Receptors, Interleukin-1; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 31430-18-9/Nocodazole
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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