| Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen, Brucella melitensis. | |
| | |
MedLine Citation:
|
PMID: 21692747 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
TIR domain containing proteins play a crucial role in innate immunity in eukaryotes. Brucella is a highly infectious intracellular bacterium that encodes a TIR domain protein (TcpB) to subvert host innate immune responses to establish a beneficial niche for pathogenesis. TcpB inhibits NF-κB activation and pro-inflammatory cytokine secretions mediated by Toll like receptor (TLR) 2 & 4. Here, we demonstrate that TcpB modulates microtubule dynamics by acting as a stabilization factor. TcpB increased the rate of nucleation as well as the polymerization phases of microtubule formation similar to paclitaxel. TcpB could efficiently inhibit nocodazole or cold induced microtubule disassembly. Microtubule stabilization by TcpB is attributed to the BB-loop region of the TIR domain and a point mutation affected the microtubule stabilization as well as the TLR suppression properties of TcpB. |
| | |
Authors:
|
Girish Radhakrishnan; Jerome Harms; Gary Splitter |
Related Documents
:
|
16608067 - The expression of maxik channel alpha--subunit during human macrophages differentiating... 377487 - Human macrophage migration inhibition factor: evidence for subunit structure. 11694237 - Inhibitory effects of rosa roxburghii tratt juice on in vitro oxidative modification of... 11978887 - Presence of macrophage migration inhibitory factor in human milk: evidence in the aqueo... 21206757 - Presenilin 2 is the predominant γ-secretase in microglia and modulates cytokine release. 16402107 - The attraction of chemokines as a target for specific anti-inflammatory therapy. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-20 |
Journal Detail:
|
Title: The Biochemical journal Volume: - ISSN: 1470-8728 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-neoplastic action of aspirin against a T cell lymphoma involves alteration in tumor microenviro...
Next Document: The site of RanGTP generation can act as an organizational cue for mitotic microtubules.