Document Detail


Modulation of microRNA associated with ovarian cancer cells by genistein.
MedLine Citation:
PMID:  20099489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Role of microRNAs in malignancies is well established due their regulatory role in cellular differentiation, proliferation and cell cycle control. Our purpose was to determine miRNA profiles of serially established ovarian cancer cell lines and the effect of genistein treatment. METHODS: Cell lines (UL-3A, UL-3B) were established from one patient during progression of disease. miRNA profiling was performed in untreated and genistein-treated cells. Estrogen receptors (ER) were studied with real-time polymerase chain reaction (RT-PCR) and Western immunoblotting. In vitro migration and invasion assays were utilized. RESULTS: While 108 miRNAs were expressed equally in both cell lines and their genistein-treated counterparts, an additional 53 miRNAs were differentially expressed. Genistein resulted in induction of ERalpha and ERbeta in ovarian cancer cells. A significant reduction in migration and invasion of UL-3A and UL-3B was demonstrated in genistein-treated cells. CONCLUSION: Common and unique miRNA profiles were demonstrated between the two cell lines, some of which were altered by genistein.
Authors:
L P Parker; D D Taylor; J Kesterson; D S Metzinger; C Gercel-Taylor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of gynaecological oncology     Volume:  30     ISSN:  0392-2936     ISO Abbreviation:  Eur. J. Gynaecol. Oncol.     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-26     Completed Date:  2010-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100357     Medline TA:  Eur J Gynaecol Oncol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  616-21     Citation Subset:  IM    
Affiliation:
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Women's Health University of Louisville School of Medicine, Louisville, KY 40202, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Estrogen Receptor alpha / metabolism
Estrogen Receptor beta / metabolism
Female
Genistein / pharmacology*
Humans
MicroRNAs / drug effects*
Ovarian Neoplasms / drug therapy*
Protein Kinase Inhibitors / pharmacology*
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/MicroRNAs; 0/Protein Kinase Inhibitors; 446-72-0/Genistein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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