Document Detail


Modulation of membrane transport by free fatty acids: inhibition of synaptosomal sodium-dependent amino acid uptake.
MedLine Citation:
PMID:  6849898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-affinity, Na+-dependent synaptosomal amino acid uptake systems are strongly stimulated by proteins which are known to bind free fatty acids. The rate of uptake as well as the overall level of accumulation is increased by such proteins as bovine serum albumin, hepatic fatty acid binding protein, beta-lactoglobulin, and fetuin. Such a stimulation is not observed with proteins which do not bind fatty acids. The transport activity of synaptosomal preparations can be directly correlated with the free fatty acid content of the preparation. Thus, incubation with albumin reduces the free fatty acid content of synaptosomal preparations, suggesting that the stimulatory effects of the proteins are related to their removal of inhibitory fatty acids formed by hydrolysis of membrane lipids during incubation. Inhibition of amino acid uptake is seen with most cis-unsaturated long chain fatty acids while saturated and trans-unsaturated fatty acids have relatively little or no effect. Under conditions in which the ionophore gramicidin D causes an increase of 22Na flux into synaptosomes, oleic acid (50 microM) has no effect on the influx. These data are consistent with the hypothesis proposed earlier by us [Rhoads, D. E., Peterson, N. A., & Raghupathy, E. (1982) Biochemistry 21, 4782] that Na+-dependent amino acid transport carrier proteins reside in a relatively fluid lipid domain in the synaptosomal membrane and that the effects of cis-unsaturated fatty acids are mediated by interactions with such domains.
Authors:
D E Rhoads; R K Ockner; N A Peterson; E Raghupathy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  22     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-07-15     Completed Date:  1983-07-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1965-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Biological Transport / drug effects
Cerebral Cortex / metabolism
Fatty Acids, Nonesterified / pharmacology*
Kinetics
Male
Rats
Rats, Inbred Strains
Serum Albumin, Bovine / pharmacology
Sodium / pharmacology*
Structure-Activity Relationship
Synaptosomes / drug effects,  metabolism*
Grant Support
ID/Acronym/Agency:
AM 13328/AM/NIADDK NIH HHS; AM 26593/AM/NIADDK NIH HHS; NS 15659/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids, Nonesterified; 0/Serum Albumin, Bovine; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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