| Modulation of matrix metalloproteinase-9 activity by hyaluronan is dependent on NF-kappaB activity in lymphoma cell lines with dissimilar invasive behavior. | |
| | |
MedLine Citation:
|
PMID: 15474489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Expression and activity of matrix metalloproteinase-9 (MMP-9) as well as its relationship with hyaluronan (HA) and NF-kappaB activity were analyzed in two murine lymphoma cell lines with dissimilar migration and invasive behavior. MMP activity was evaluated by zymograms in supernatants, membrane extracts of tumor cells, and in the organs invaded by these cells. The more aggressive LBLa cell line showed MMP-9 activity in vitro, which increased after HA treatment and was blocked by anti-CD44 mAb. Such activity was not found in the less aggressive LBLc. MMP-9 and MMP-2 activity was found in organs invaded by both cell lines, although differential MMP-9 activity was observed in lung infiltrated only by LBLa cell line. NF-kappaB activation was evaluated to determine whether differential activity of MMP-9 was dependent on downstream signaling pathway, showing higher NF-kappaB activity in the more aggressive LBLa cell line. Our results showed that MMP-9 activity modulated by HA through NF-kappaB signaling pathway may be involved in the aggressive behavior of LBLa. |
| | |
Authors:
|
Laura Alaniz; Mariana García; Paula Cabrera; María Arnaiz; Victoria Cavaliere; Guillermo Blanco; Elida Alvarez; Silvia Hajos |
Related Documents
:
|
16364029 - Expression of matrix metalloproteinase (mmp)-2, mmp-9, mmp-13, and mt1-mmp in skin tumo... 17452979 - An osteopontin fragment is essential for tumor cell invasion in hepatocellular carcinoma. 23387899 - Selective carbonic anhydrase ix inhibitors based on coumarin scaffold as promising anti... 16680569 - Matrix metalloproteinases and tumor metastasis. 8567119 - High level of mt-mmp expression is associated with invasiveness of cervical cancer cells. 10770639 - Reduction of breast carcinoma tumor growth and lung colonization by overexpression of t... 9130989 - Salivary gland-like tumors of the sellar region. 20381449 - The pivotal roles of mitochondria in cancer: warburg and beyond and encouraging prospec... 17183069 - Genetic and epigenetic profiling in early colorectal tumors and prediction of invasive ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 324 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2004 Nov |
Date Detail:
|
Created Date: 2004-10-11 Completed Date: 2004-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 736-43 Citation Subset: IM |
Affiliation:
|
Cátedra de Inmunología-IDEHU, Facultad de Farmacia y Bioquímica, UBA-CONICET, Junín 956, piso 4, CP: 1113, Buenos Aires, Argentina. laulaniz@ffyb.uba.ar |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD44 / biosynthesis, metabolism Blotting, Western Cell Line Cell Line, Tumor Cell Membrane / metabolism Cell Nucleus / metabolism DNA, Complementary / metabolism Hyaluronic Acid / pharmacology* Lymphoma / metabolism* Male Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism* Mice Mice, Inbred BALB C NF-kappa B / metabolism* Neoplasm Invasiveness Polymerase Chain Reaction Recombinant Proteins / chemistry Reverse Transcriptase Polymerase Chain Reaction Signal Transduction |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD44; 0/DNA, Complementary; 0/NF-kappa B; 0/Recombinant Proteins; 9004-61-9/Hyaluronic Acid; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of residual structure within denatured antichymotrypsin: implications for serpin fold...
Next Document: Toxoplasma gondii exposes phosphatidylserine inducing a TGF-beta1 autocrine effect orchestrating mac...