| Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA interference. | |
| | |
MedLine Citation:
|
PMID: 23148270 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Truncated light-harvesting antenna 1 (TLA1) is a nuclear gene proposed to regulate the chlorophyll (Chl) antenna size in Chlamydomonas reinhardtii. The Chl antenna size of the photosystems and the chloroplast ultrastructure were manipulated upon TLA1 gene over-expression and RNAi downregulation. The TLA1 over-expressing lines possessed a larger chlorophyll antenna size for both photosystems and contained greater levels of Chl b per cell relative to the wild type. Conversely, TLA1 RNAi transformants had a smaller Chl antenna size for both photosystems and lower levels of Chl b per cell. Western blot analyses of the TLA1 over-expressing and RNAi transformants showed that modulation of TLA1 gene expression was paralleled by modulation in the expression of light-harvesting protein, reaction centre D1 and D2, and VIPP1 genes. Transmission electron microscopy showed that modulation of TLA1 gene expression impacts the organization of thylakoid membranes in the chloroplast. Over-expressing lines showed well-defined grana, whereas RNAi transformants possessed loosely held together and more stroma-exposed thylakoids. Cell fractionation suggested localization of the TLA1 protein in the inner chloroplast envelope and potentially in association with nascent thylakoid membranes, indicating a role in Chl antenna assembly and thylakoid membrane biogenesis. The results provide a mechanistic understanding of the Chl antenna size regulation by the TLA1 gene. |
| | |
Authors:
|
Mautusi Mitra; Henning Kirst; David Dewez; Anastasios Melis |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Philosophical transactions of the Royal Society of London. Series B, Biological sciences Volume: 367 ISSN: 1471-2970 ISO Abbreviation: Philos. Trans. R. Soc. Lond., B, Biol. Sci. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2012-11-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7503623 Medline TA: Philos Trans R Soc Lond B Biol Sci Country: England |
Other Details:
|
Languages: eng Pagination: 3430-43 Citation Subset: IM |
Affiliation:
|
Department of Plant and Microbial Biology, University of California, , Berkeley, CA 94720, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulatory factors for the assembly of thylakoid membrane protein complexes.
Next Document: Subunit composition of CP43-less photosystem II complexes of Synechocystis sp. PCC 6803: implication...