Document Detail


Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA interference.
MedLine Citation:
PMID:  23148270     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Truncated light-harvesting antenna 1 (TLA1) is a nuclear gene proposed to regulate the chlorophyll (Chl) antenna size in Chlamydomonas reinhardtii. The Chl antenna size of the photosystems and the chloroplast ultrastructure were manipulated upon TLA1 gene over-expression and RNAi downregulation. The TLA1 over-expressing lines possessed a larger chlorophyll antenna size for both photosystems and contained greater levels of Chl b per cell relative to the wild type. Conversely, TLA1 RNAi transformants had a smaller Chl antenna size for both photosystems and lower levels of Chl b per cell. Western blot analyses of the TLA1 over-expressing and RNAi transformants showed that modulation of TLA1 gene expression was paralleled by modulation in the expression of light-harvesting protein, reaction centre D1 and D2, and VIPP1 genes. Transmission electron microscopy showed that modulation of TLA1 gene expression impacts the organization of thylakoid membranes in the chloroplast. Over-expressing lines showed well-defined grana, whereas RNAi transformants possessed loosely held together and more stroma-exposed thylakoids. Cell fractionation suggested localization of the TLA1 protein in the inner chloroplast envelope and potentially in association with nascent thylakoid membranes, indicating a role in Chl antenna assembly and thylakoid membrane biogenesis. The results provide a mechanistic understanding of the Chl antenna size regulation by the TLA1 gene.
Authors:
Mautusi Mitra; Henning Kirst; David Dewez; Anastasios Melis
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Philosophical transactions of the Royal Society of London. Series B, Biological sciences     Volume:  367     ISSN:  1471-2970     ISO Abbreviation:  Philos. Trans. R. Soc. Lond., B, Biol. Sci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-13     Completed Date:  2013-04-22     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  7503623     Medline TA:  Philos Trans R Soc Lond B Biol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  3430-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Nucleus / genetics,  metabolism
Chlamydomonas reinhardtii / genetics,  metabolism*
Chlorophyll / genetics,  metabolism
Chloroplast Proteins / genetics,  metabolism
Chloroplasts / genetics,  metabolism,  ultrastructure
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Plant*
Genes, Plant*
Light-Harvesting Protein Complexes / genetics,  metabolism*
Microscopy, Electron, Transmission
Organelle Size*
Plant Cells / metabolism
RNA Interference*
Thylakoids / metabolism
Chemical
Reg. No./Substance:
0/Chloroplast Proteins; 0/Light-Harvesting Protein Complexes; 1406-65-1/Chlorophyll; 5712ZB110R/chlorophyll b
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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