Document Detail

Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous alpha-amylase in the diet.
MedLine Citation:
PMID:  19343525     Owner:  NLM     Status:  MEDLINE    
A 60-day feeding trial was conducted to delineate the effect of both gelatinized (G) and non-gelatinized (NG) corn with or without supplementation of exogenous alpha-amylase, either at optimum (35%) or sub-optimum (27%) protein levels, on blood glucose, and the key metabolic enzymes of glycolysis (hexokinase, HK), gluconeogenesis (glucose-6 phosphatase, G6Pase and fructose-1,6 bisphosphatase, FBPase), lipogenesis (glucose-6 phosphate dehydrogenase, G6PD) and amino acid metabolism (alanine amino transferase, ALT and aspartate amino transferase, AST) in Labeo rohita. Three hundred and sixty juveniles (average weight 10 +/- 0.15 g) were randomly distributed into 12 treatment groups with each of two replicates. Twelve semi-purified diets containing either 35 or 27% crude protein were prepared by including G or NG corn as carbohydrate source with different levels of microbial alpha-amylase (0, 50, 100 and 150 mg kg(-1)). The G corn fed groups showed significantly higher (P < 0.05) blood glucose and G6PD activity, whereas G6Pase, FBPase, ALT and AST activity in liver was higher in the NG corn fed group. Dietary corn type, alpha-amylase level in diet or their interaction had no significant effect (P > 0.05) on liver HK activity, but the optimum crude protein (35%) fed group showed higher HK activity than their low protein counterparts. The sub-optimum crude protein (27%) fed group showed significantly higher (P < 0.05) G6PD activity than the optimum protein fed group, whereas the reverse trend was observed for HK, G6Pase, FBPase, ALT and AST activity. Addition of 50 mg alpha-amylase kg(-1) feed showed increased blood glucose and G6PD activity of the NG corn fed group, whereas the reverse trend was found for G6Pase, FBPase, ALT and AST activity in liver, which was similar to that of the G or NG corn supplemented with 100/150 mg alpha-amylase kg(-1) feed. Data on enzyme activities suggest that NG corn in the diet significantly induced more gluconeogenic and amino acid metabolic enzyme activity, whereas G corn induced increased lipogenic enzyme activity. Increased amino acid catabolic enzyme (ALT and AST) activity was observed either at optimum protein (35%) irrespective of corn type or NG corn without supplementation of alpha-amylase irrespective of protein level in the diet.
Shivendra Kumar; N P Sahu; A K Pal; Vidya Sagar; Amit Kumar Sinha; Kartik Baruah
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Publication Detail:
Type:  Journal Article     Date:  2008-04-04
Journal Detail:
Title:  Fish physiology and biochemistry     Volume:  35     ISSN:  1573-5168     ISO Abbreviation:  Fish Physiol. Biochem.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-04-03     Completed Date:  2009-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955049     Medline TA:  Fish Physiol Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  301-15     Citation Subset:  IM    
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
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MeSH Terms
Blood Glucose / analysis
Cyprinidae / physiology*
Diet / veterinary*
Dietary Carbohydrates / administration & dosage,  metabolism*
Dietary Proteins / administration & dosage,  metabolism*
Enzymes / metabolism*
Liver / enzymology
Muscles / enzymology
Random Allocation
Starch / metabolism
alpha-Amylases / administration & dosage,  metabolism*
Reg. No./Substance:
0/Blood Glucose; 0/Dietary Carbohydrates; 0/Dietary Proteins; 0/Enzymes; 9005-25-8/Starch; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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