| Modulation of interleukin-2-driven proliferation of human large granular lymphocytes by carbaryl, an anticholinesterase insecticide. | |
| | |
MedLine Citation:
|
PMID: 1916080 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Studies in other laboratories have provided evidence that the interleukin-2 (IL2) signaling pathway in lymphocytes includes essential, serine proteases. Since the anticholinesterase (antiCHE) insecticides are potent serine hydrolase (esterase and protease) inhibitors, we assessed the ability of carbaryl (CA, a widely used antiCHE insecticide) and alpha-naphthol (NA, a major metabolite of carbaryl) to modulate IL2-driven proliferation of large granular lymphocytes (LGL) purified from human peripheral blood. These cells express nearly all of the natural killer (NK) activity of human peripheral blood. NK cells are normal lymphocytes that respond to IL2 by proliferating and increasing their tumoricidal activity on a per cell basis. Cultures of purified LGL, initiated in the presence of human recombinant IL2, were harvested on culture Day 4, then proliferation was measured as [3H]thymidine incorporation. When added only at the time cultures were initiated. CA inhibited incorporation 10-32%, 35-53%, and 54-57% at 0.5, 5.0, and 50 microM, respectively. In contrast, NA had no effect at 0.5 and 5.0 microM, but was inhibitory (16-17%) at 50 microM. Reexposure to CA or NA, during the incorporation assay, had little effect on the observed inhibition profiles. Chemically induced changes in cell number during an 8-day culture period reflect the chemically induced changes in [3H]thymidine incorporation. Neither CA nor NA produced cell death. Quantitation of both CA and NA by HPLC indicated a rapid loss of CA (ca. 95% in 24 hr) and a much slower loss of NA from the culture medium. CA inhibited human LGL proliferation at concentrations producing little or no inhibition of serum CHE, an indicator of exposure to antiCHE insecticides. |
| | |
Authors:
|
S Bavari; G P Casale; R E Gold; E F Vitzthum |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Fundamental and applied toxicology : official journal of the Society of Toxicology Volume: 17 ISSN: 0272-0590 ISO Abbreviation: Fundam Appl Toxicol Publication Date: 1991 Jul |
Date Detail:
|
Created Date: 1991-11-13 Completed Date: 1991-11-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8200838 Medline TA: Fundam Appl Toxicol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 61-74 Citation Subset: IM |
Affiliation:
|
Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha 68198-6025. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Carbaryl / toxicity* Cell Adhesion / drug effects Cell Death / drug effects Cell Division / physiology* Humans Interleukin-2 / physiology* Killer Cells, Natural / drug effects Lymphocytes / drug effects* Male Naphthols / toxicity Thymidine / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Interleukin-2; 0/Naphthols; 50-89-5/Thymidine; 63-25-2/Carbaryl; 90-15-3/1-naphthol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chronic exposure to a simulated urban profile of ozone alters ventilatory responses to carbon dioxid...
Next Document: Investigations of amitraz neurotoxicity in rats. IV. Assessment of toxicity syndrome using a functio...