Document Detail


Modulation of inflammatory response after spinal cord trauma with deferoxamine, an iron chelator.
MedLine Citation:
PMID:  20370565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The standard iron-chelator deferoxamine is known to reduce neurological deficits. The aim of the present study was to evaluate the contribution of deferoxamine in the secondary damage in experimental spinal cord injury (SCI) in mice, induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy. SCI resulted in production of inflammatory mediators, tissue damage and apoptosis. Deferoxamine treatment 30 min before and 1 and 6 h after the SCI significantly reduced: (1) GFAP immunoreactivity, (2) neutrophil infiltration, (3) NF-kappaB activation, (4) iNOS expression, (5) nitrotyrosine and MDA formation, (6) DNA damage (methyl green pyronin staining and PAR formation and (7) apoptosis (TUNEL staining, FasL, Bax and Bcl-2 expression, S-100 expression). Moreover, deferoxamine significantly ameliorated the recovery of limb function (evaluated by motor recovery score). Taken together, the results clearly demonstrate that deferoxamine treatment reduces the development of inflammation and tissue injury associated with spinal cord trauma.
Authors:
Irene Paterniti; Emanuela Mazzon; Esposito Emanuela; Rosanna Di Paola; Maria Galuppo; Placido Bramanti; Salvatore Cuzzocrea
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  44     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  694-709     Citation Subset:  IM    
Affiliation:
Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, Gazzi, Messina, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Blotting, Western
Deferoxamine / therapeutic use*
Immunohistochemistry
In Situ Nick-End Labeling
Inflammation / etiology,  prevention & control*
Inflammation Mediators / metabolism
Iron Chelating Agents / therapeutic use*
Male
Mice
Neutrophil Infiltration / drug effects
Recovery of Function / drug effects
Spinal Cord Injuries / complications,  drug therapy*,  pathology
Chemical
Reg. No./Substance:
0/Inflammation Mediators; 0/Iron Chelating Agents; 70-51-9/Deferoxamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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