| Modulation of immune responses to bovine herpesvirus-1 in cattle by immunization with a DNA vaccine encoding glycoprotein D as a fusion protein with bovine CD154. | |
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MedLine Citation:
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PMID: 15147576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to determine whether a DNA vaccine encoding bovine CD154 linked to a truncated version of bovine herpesvirus-1 (BHV-1) glycoprotein D (tgD-CD154) induces enhanced tgD-specific immune responses in cattle. In vitro characterization demonstrated that tgD and tgD-CD154 both bind to cultured bovine B cells, whereas only tgD-CD154 induces interleukin-4-dependent proliferation, suggesting that tgD-CD154 specifically binds the CD40 receptor and induces receptor signalling. Calves were immunized with plasmid encoding either tgD or tgD-CD154 by intradermal injection with a needle-free device. After two immunizations, tgD-specific immune responses were observed in both vaccinated groups and after challenge with BHV-1 these responses further increased. Animals immunized with plasmid encoding tgD-CD154 had significantly higher tgD-specific serum titres of immunoglobulins G and A but significantly lower numbers of tgD-specific interferon-gamma-secreting cells than animals immunized with plasmid encoding tgD after BHV-1 challenge. This suggests that the expression of an antigen as a chimeric protein with CD154 can qualitatively alter immune responses in cattle. Since we previously showed that plasmid encoding tgD-CD154 induces significantly enhanced secondary tgD-specific antibody responses in sheep, there appear to be interspecies differences in the immune responses induced by tgD-CD154, which suggests that both proteins in the chimeric molecule may influence protein targeting and the induction of an immune response. |
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Authors:
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Sharmila Manoj; Philip J Griebel; Lorne A Babiuk; Sylvia van Drunen Littel-van den Hurk |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Immunology Volume: 112 ISSN: 0019-2805 ISO Abbreviation: Immunology Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-05-18 Completed Date: 2004-06-25 Revised Date: 2013-06-09 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: England |
Other Details:
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Languages: eng Pagination: 328-38 Citation Subset: IM |
Affiliation:
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Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral / biosynthesis Antigens, Viral / immunology CD40 Ligand / immunology Cattle / immunology* Cattle Diseases / prevention & control Cells, Cultured Herpesviridae Infections / prevention & control, veterinary Herpesvirus 1, Bovine / immunology* Herpesvirus Vaccines / immunology* Immunity, Cellular Immunization Recombinant Fusion Proteins / immunology Transfection Vaccines, DNA / immunology* Viral Proteins / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Antigens, Viral; 0/Herpesvirus Vaccines; 0/Recombinant Fusion Proteins; 0/Vaccines, DNA; 0/Viral Proteins; 0/bovine herpesvirus type-1 glycoproteins; 147205-72-9/CD40 Ligand |
| Comments/Corrections | |
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