Document Detail


Modulation of immune responses to bovine herpesvirus-1 in cattle by immunization with a DNA vaccine encoding glycoprotein D as a fusion protein with bovine CD154.
MedLine Citation:
PMID:  15147576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to determine whether a DNA vaccine encoding bovine CD154 linked to a truncated version of bovine herpesvirus-1 (BHV-1) glycoprotein D (tgD-CD154) induces enhanced tgD-specific immune responses in cattle. In vitro characterization demonstrated that tgD and tgD-CD154 both bind to cultured bovine B cells, whereas only tgD-CD154 induces interleukin-4-dependent proliferation, suggesting that tgD-CD154 specifically binds the CD40 receptor and induces receptor signalling. Calves were immunized with plasmid encoding either tgD or tgD-CD154 by intradermal injection with a needle-free device. After two immunizations, tgD-specific immune responses were observed in both vaccinated groups and after challenge with BHV-1 these responses further increased. Animals immunized with plasmid encoding tgD-CD154 had significantly higher tgD-specific serum titres of immunoglobulins G and A but significantly lower numbers of tgD-specific interferon-gamma-secreting cells than animals immunized with plasmid encoding tgD after BHV-1 challenge. This suggests that the expression of an antigen as a chimeric protein with CD154 can qualitatively alter immune responses in cattle. Since we previously showed that plasmid encoding tgD-CD154 induces significantly enhanced secondary tgD-specific antibody responses in sheep, there appear to be interspecies differences in the immune responses induced by tgD-CD154, which suggests that both proteins in the chimeric molecule may influence protein targeting and the induction of an immune response.
Authors:
Sharmila Manoj; Philip J Griebel; Lorne A Babiuk; Sylvia van Drunen Littel-van den Hurk
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Immunology     Volume:  112     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-18     Completed Date:  2004-06-25     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  328-38     Citation Subset:  IM    
Affiliation:
Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Viral / biosynthesis
Antigens, Viral / immunology
CD40 Ligand / immunology
Cattle / immunology*
Cattle Diseases / prevention & control
Cells, Cultured
Herpesviridae Infections / prevention & control,  veterinary
Herpesvirus 1, Bovine / immunology*
Herpesvirus Vaccines / immunology*
Immunity, Cellular
Immunization
Recombinant Fusion Proteins / immunology
Transfection
Vaccines, DNA / immunology*
Viral Proteins / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/Herpesvirus Vaccines; 0/Recombinant Fusion Proteins; 0/Vaccines, DNA; 0/Viral Proteins; 0/bovine herpesvirus type-1 glycoproteins; 147205-72-9/CD40 Ligand
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