Document Detail


Modulation of hybridoma formation by dexamethasone.
MedLine Citation:
PMID:  3724767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In an effort to determine the effect of dexamethasone on hybridoma formation, spleen cells from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were fused with mouse plasmacytoma cells (P3U1) in the presence of polyethylene glycol (PEG). Dexamethasone was added in decreasing doses (10(-3) to 10(-9) mM) to the hypoxanthine-aminopterin-thymide (HAT) medium immediately after the PEG-mediated cell fusion. 10(-3) mM of this steroid was found to inhibit markedly the number and size of hybridoma clones generated, while 10(-5) mM dexamethasone was shown to enhance hybridoma formation. The effect of 10(-3) mM dexamethasone was most pronounced when added immediately after fusion. When this dose was given 48 or 120 h after cell fusion, the extent of the inhibitory effect was less pronounced. High concentration of dexamethasone may also inhibit monoclonal antibody production by hybridomas once generated. An increase in the number of clones formed was observed when 10(-5) mM dexamethasone was added to HAT medium as well as an increase in the average colony size. Large clones were also observed with lower dexamethasone doses ranging from 10(-7) to 10(-9) mM. Possible mechanisms on the effect of dexamethasone on hybridoma formation are discussed.
Authors:
A H Bartal; C Feit; Y Hirshaut
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Natural immunity and cell growth regulation     Volume:  5     ISSN:  0254-7600     ISO Abbreviation:  Nat. Immun. Cell Growth Regul.     Publication Date:  1986  
Date Detail:
Created Date:  1986-08-08     Completed Date:  1986-08-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8407979     Medline TA:  Nat Immun Cell Growth Regul     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  107-12     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Formation / drug effects
Cell Fusion / drug effects
Culture Media
Dexamethasone / pharmacology*
Dose-Response Relationship, Drug
Female
Hybridomas / drug effects*
Mice
Mice, Inbred BALB C
Grant Support
ID/Acronym/Agency:
CA-32245/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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