| Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp. | |
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MedLine Citation:
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PMID: 19022329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tamarind (Tamarindus indica L.) is indigenous to Asian countries and widely cultivated in the American continents. The tamarind fruit pulp extract (ExT), traditionally used in spices, food components and juices, is rich in polyphenols that have demonstrated anti-atherosclerotic, antioxidant and immunomodulatory activities. This study evaluated the modulator effect of a crude hydroalcoholic ExT on some peripheral human neutrophil functions. The neutrophil reactive oxygen species generation, triggered by opsonized zymosan (OZ), n-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), and assessed by luminol- and lucigenin-enhanced chemiluminescence (LumCL and LucCL, respectively), was inhibited by ExT in a concentration-dependent manner. ExT was a more effective inhibitor of the PMA-stimulated neutrophil function [IC50 (in microg/10(6)cells)=115.7+/-9.7 (LumCL) and 174.5+/-25.9 (LucCL)], than the OZ- [IC50=248.5+/-23.1 (LumCL) and 324.1+/-34.6 (LucCL)] or fMLP-stimulated cells [IC50=178.5+/-12.2 (LumCL)]. The ExT also inhibited neutrophil NADPH oxidase activity (evaluated by O2 consumption), degranulation and elastase activity (evaluated by spectrophotometric methods) at concentrations higher than 200 microg/10(6)cells, without being toxic to the cells, under the conditions assessed. Together, these results indicate the potential of ExT as a source of compounds that can modulate the neutrophil-mediated inflammatory diseases. |
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Authors:
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Fabiana S Paula; Luciana M Kabeya; Alexandre Kanashiro; Andréa S G de Figueiredo; Ana Elisa C S Azzolini; Sérgio A Uyemura; Yara Maria Lucisano-Valim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-03 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 47 ISSN: 1873-6351 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-12 Completed Date: 2009-03-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 163-70 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Avenida Bandeirantes n 3900, CEP 14049-900, Ribeirão Preto, SP, Brazil. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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chemistry,
pharmacology Cell Degranulation / drug effects Cells, Cultured Chemiluminescent Measurements Female Fruit / chemistry* Humans Male NADPH Oxidase / antagonists & inhibitors Neutrophils / drug effects*, metabolism* Pancreatic Elastase / antagonists & inhibitors Phorbol Esters Plant Extracts / chemistry, pharmacology* Reactive Oxygen Species / metabolism Tamarindus / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Phorbol Esters; 0/Plant Extracts; 0/Reactive Oxygen Species; 24928-15-2/phorbol-12,13-diacetate; EC 1.6.3.1/NADPH Oxidase; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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