Document Detail

Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp.
MedLine Citation:
PMID:  19022329     Owner:  NLM     Status:  MEDLINE    
The tamarind (Tamarindus indica L.) is indigenous to Asian countries and widely cultivated in the American continents. The tamarind fruit pulp extract (ExT), traditionally used in spices, food components and juices, is rich in polyphenols that have demonstrated anti-atherosclerotic, antioxidant and immunomodulatory activities. This study evaluated the modulator effect of a crude hydroalcoholic ExT on some peripheral human neutrophil functions. The neutrophil reactive oxygen species generation, triggered by opsonized zymosan (OZ), n-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), and assessed by luminol- and lucigenin-enhanced chemiluminescence (LumCL and LucCL, respectively), was inhibited by ExT in a concentration-dependent manner. ExT was a more effective inhibitor of the PMA-stimulated neutrophil function [IC50 (in microg/10(6)cells)=115.7+/-9.7 (LumCL) and 174.5+/-25.9 (LucCL)], than the OZ- [IC50=248.5+/-23.1 (LumCL) and 324.1+/-34.6 (LucCL)] or fMLP-stimulated cells [IC50=178.5+/-12.2 (LumCL)]. The ExT also inhibited neutrophil NADPH oxidase activity (evaluated by O2 consumption), degranulation and elastase activity (evaluated by spectrophotometric methods) at concentrations higher than 200 microg/10(6)cells, without being toxic to the cells, under the conditions assessed. Together, these results indicate the potential of ExT as a source of compounds that can modulate the neutrophil-mediated inflammatory diseases.
Fabiana S Paula; Luciana M Kabeya; Alexandre Kanashiro; Andréa S G de Figueiredo; Ana Elisa C S Azzolini; Sérgio A Uyemura; Yara Maria Lucisano-Valim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-03
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  47     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-12     Completed Date:  2009-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  163-70     Citation Subset:  IM    
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Avenida Bandeirantes n 3900, CEP 14049-900, Ribeirão Preto, SP, Brazil.
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MeSH Terms
Antioxidants / chemistry,  pharmacology
Cell Degranulation / drug effects
Cells, Cultured
Chemiluminescent Measurements
Fruit / chemistry*
NADPH Oxidase / antagonists & inhibitors
Neutrophils / drug effects*,  metabolism*
Pancreatic Elastase / antagonists & inhibitors
Phorbol Esters
Plant Extracts / chemistry,  pharmacology*
Reactive Oxygen Species / metabolism
Tamarindus / chemistry*
Reg. No./Substance:
0/Antioxidants; 0/Phorbol Esters; 0/Plant Extracts; 0/Reactive Oxygen Species; 24928-15-2/phorbol-12,13-diacetate; EC Oxidase; EC Elastase

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