Document Detail

Modulation of human lymphocyte proliferation by amino acids.
MedLine Citation:
PMID:  2379319     Owner:  NLM     Status:  MEDLINE    
The amino acids required for phytohaemagglutinin (PHA) induced lymphocyte proliferation were determined by the 3H-thymidine incorporation in amino acid-deficient media. Results indicate that the PHA-stimulated lymphocytes require alanine and serine in addition to 13 other amino acids present in Eagle's minimal essential medium (arginine, cysteine, glutamine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, tyrosine, and valine). The omission of any one of the 13 amino acids would stop almost completely the proliferation of PHA-stimulated lymphocytes. The omission of serine from RPMI 1640 medium caused a mean reduction of 64% of cell proliferation, while the addition of alanine to PRMI 1640 culture medium caused a mean increment of 52%. The lymphocyte proliferation appears to be modulated by amino acids in the culture medium, and for optimal growth of lymphocytes, all these 15 amino acids are essential.
J C Chuang; C L Yu; S R Wang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  81     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-09-11     Completed Date:  1990-09-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  173-6     Citation Subset:  IM    
Department of Medicine, Veterans General Hospital, Taipei, Taiwan, Republic of China.
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MeSH Terms
Amino Acids / physiology*
Cells, Cultured
Culture Media
Lymphocyte Activation*
Lymphocytes / physiology*
Phytohemagglutinins / pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Phytohemagglutinins

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