Document Detail


Modulation of cytarabine metabolism in the human promyelocytic leukemia cell line HL-60 by polyhydroxy-substituted benzohydroxamic acids.
MedLine Citation:
PMID:  6957264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two potent new ribonucleotide reductase inhibitors, 3,4,5-trihydroxybenzohydroxamic acid (VF 122) and 3,4-dihydroxybenzohydroxamic acid (VF 147), were investigated for their ability to modulate the cellular pharmacology of cytarabine (ara-C) in HL-60 cells. VF 122 and VF 147 increased the total cellular uptake of ara-C by a mean (+/- SE) of 8% +/- 3% and 29% +/- 3%, respectively, when measured 2 hours after the start of exposure to 0.1 microM ara-C. This effect was evident after only 10 minutes of exposure to the riboNucleotide reductase inhibitor and did not vary significantly over the concentration range of 10-100 microM for either agent. VF 122 enhanced the incorporation of ara-CTP into DNA by 3.6-fold; VF 147 produced a 5.6-fold increase. In comparison, the maximum enhancement achievable with hydroxyurea was 2.1-fold, and with thymidine was 1.8-fold. These results provide a biochemical rationale for further investigation of these agents in combination with ara-C.
Authors:
S B Howell; S Gill; H L Elford
Related Documents :
2866244 - Nutritional requirements of plasmodium falciparum in culture. iii. further observations...
12616154 - The effects of folic acid in the prevention of neural tube development defects caused b...
97234 - Adjuvant activity of synthetic 6-o-"mycoloyl"-n-acetylmuramyl-l-alanyl-d-isoglutamine a...
11718574 - Carrier-mediated transport of folic acid in bewo cell monolayers as a model of the huma...
9038424 - 3-hydroxymethylphenytoin valproic acid ester, a new prodrug combining two anticonvulsan...
7165554 - Utilization of bicarbonate for base repletion in hemodialysis.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer treatment reports     Volume:  66     ISSN:  0361-5960     ISO Abbreviation:  Cancer Treat Rep     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1982-12-16     Completed Date:  1982-12-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7607107     Medline TA:  Cancer Treat Rep     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1825-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line
Cytarabine / metabolism*
DNA / metabolism
Humans
Hydroxamic Acids / pharmacology*
Leukemia, Myeloid / metabolism*
Time Factors
Grant Support
ID/Acronym/Agency:
CA-25336/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Hydroxamic Acids; 147-94-4/Cytarabine; 69839-82-3/3,4,5-trihydroxybenzohydroxamic acid; 69839-83-4/3,4-dihydroxybenzohydroxamic acid; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sensitivity of cultured human osteosarcoma and chondrosarcoma cells to retinoic acid.
Next Document:  Comparison of the contraction produced by various tryptamine analogues on human basilar arterial and...