| Modulation of coronavirus-mediated cell fusion by homeostatic control of cholesterol and fatty acid metabolism. | |
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MedLine Citation:
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PMID: 1662706 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cellular susceptibility to fusion mediated by murine coronavirus (mouse hepatitis virus, MHV strain A59) was separated into lipid-dependent and lipid-independent mechanisms with the use of subclones and selected mutants of mouse L-2 fibroblasts. Fusion-resistant L-2 cell mutants had similar cholesterol and fatty acid composition as did their fusion-susceptible parent subclone, and were presumably deficient in a genetically mutable non-lipid, host cell factor (e.g., fusion protein receptor). On the other hand, cellular sensitivity to virus fusion, which is known to be influenced by cell cholesterol content [Daya et al., 1988], was shown further to be modulated by homeostatic alterations in fatty acid metabolism. Cholesterol supplementation of mouse L-2 fibroblasts or of peritoneal macrophages from MHV-susceptible mice elevated susceptibility to viral fusion. Increased fusion susceptibility occurred in cholesterol-supplemented L-2 cells in the absence of any detectable alterations in host cell fatty acid composition, thus demonstrating fusion enhancement by cholesterol alone. L-2 cells cloned by limiting dilution in normal (not cholesterol-supplemented) medium were found to be heterogeneous in cholesterol content. Interestingly, high cholesterol-containing subclones had increased levels of C-18:0, C-18:2, C-20:4, and C-22:6 and markedly reduced levels of C-18:1 fatty acids when compared to low cholesterol-containing subclones. High cholesterol-containing subclones did not show enhanced susceptibility to viral fusion, suggesting that homeostatic alteration of fatty acid metabolism compensated for the increased cholesterol levels and countered the normally fusion-enhancing effect of cholesterol alone.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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M Cervin; R Anderson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medical virology Volume: 35 ISSN: 0146-6615 ISO Abbreviation: J. Med. Virol. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1992-02-14 Completed Date: 1992-02-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 142-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion Cells, Cultured Cholesterol / metabolism* Cholesterol, Dietary / pharmacology Chromatography, Thin Layer Coronaviridae / growth & development* Coronaviridae Infections / metabolism, microbiology Fatty Acids / metabolism* Fibroblasts / microbiology Homeostasis Macrophages / microbiology Mice Mice, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol, Dietary; 0/Fatty Acids; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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