Document Detail

Modulation of complement component (C3 and factor B) biosynthesis by a histone deacetylase inhibitor in human intestinal epithelial cells.
MedLine Citation:
PMID:  10851266     Owner:  NLM     Status:  MEDLINE    
Sodium butyrate enhances TNF-alpha-induced complement C3 secretion but suppresses TNF-alpha-induced factor B secretion in intestinal epithelial cells. To further evaluate the mechanism underlying these responses, we assessed the effects of trichostatin A, a compound structurally unrelated to butyrate and a potent inhibitor of histone deacetylase. The C3 and factor B secretion was evaluated by enzyme-linked immunosorbent assay (ELISA) and Northern blot, and the activation of transcription factor was assessed by an electrophoretic gel mobility shift assay (EMSA). Like sodium butyrate, trichostatin A enhanced TNF-alpha-induced C3 secretion, but suppressed TNF-alpha-induced factor B secretion. These effects were also observed at the level of mRNA. EMSAs indicated that trichostatin A weakly suppressed TNF-alpha-induced NF-kappaB and NF-IL6 activation. These observations differ from previous reports that sodium butyrate potently suppressed NF-kappaB activation but enhanced NF-IL6 activation. Trichostatin A modulated TNF-alpha-induced C3 and factor B secretion in a manner similar to that induced by sodium butyrate, suggesting that both sodium butyrate and trichostatin A exert certain counter-regulatory effects associated with histone hyperacetylation. However, it remains to be determined which factors other than histone acetylation are responsible for the counter-regulation of TNF-alpha-induced C3 and factor B gene expression.
A Andoh; Y Fujiyama; M Shimada; T Bamba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  6     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-21     Completed Date:  2000-08-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  51-4     Citation Subset:  IM    
Department of Internal Medicine, Shiga University of Medical Science, Seta-Tukinowa, Otsu 520-2192, Japan.
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MeSH Terms
Blotting, Northern
Butyric Acid / metabolism*,  pharmacology
CCAAT-Enhancer-Binding Proteins
Complement C3 / biosynthesis*,  drug effects,  secretion
Complement Factor B / biosynthesis*,  drug effects,  secretion
DNA-Binding Proteins / metabolism
Enzyme Inhibitors / pharmacology*
Epithelial Cells / drug effects,  metabolism*
Histone Deacetylase Inhibitors*
Hydroxamic Acids / pharmacology*
Intestinal Mucosa / drug effects,  metabolism*
NF-kappa B / metabolism
Nuclear Proteins / metabolism
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / metabolism
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/Complement C3; 0/DNA-Binding Proteins; 0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/NF-kappa B; 0/Nuclear Proteins; 0/Tumor Necrosis Factor-alpha; 107-92-6/Butyric Acid; 58880-19-6/trichostatin A; EC Factor B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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