Document Detail


Modulation of the cell division cycle by human papillomavirus type 18 E4.
MedLine Citation:
PMID:  12368334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The life cycle of human papillomaviruses (HPVs) is tightly coupled to the differentiation program of their host epithelial cells. HPV E4 gene expression is first observed in the parabasal layers of squamous epithelia, suggesting that the E4 gene product contributes to the mechanism of differentiation-dependent virus replication, although its biological function remains unclear. We analyzed the effect of HPV type 18 E4 on cell proliferation and found that E4 expression induced cell cycle arrest at the G(2)/M boundary. The functional region of E4 necessary for the growth arrest activity was located in the central portion of the molecule, and this activity was independent of the E4-mediated collapse of cytokeratin intermediate filament structures.
Authors:
Tomomi Nakahara; Akiko Nishimura; Masakazu Tanaka; Takaharu Ueno; Akinori Ishimoto; Hiroyuki Sakai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  76     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-07     Completed Date:  2002-11-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10914-20     Citation Subset:  IM    
Affiliation:
Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-Ku, Kyoto 606-8507, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Division
Cell Line
Cercopithecus aethiops
G2 Phase
Hela Cells
Humans
Keratins / metabolism
Mitosis
Oncogene Proteins, Viral / genetics,  metabolism*
Papillomaviridae / metabolism*
Chemical
Reg. No./Substance:
0/Oncogene Proteins, Viral; 0/oncogene protein E1, Human papillomavirus type 18; 0/oncogene protein E4, Human papillomavirus type 18; 68238-35-7/Keratins
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