Document Detail

Modulation of the c-myb, c-myc and p53 mRNA and protein levels during induced murine erythroleukemia cell differentiation.
MedLine Citation:
PMID:  2648254     Owner:  NLM     Status:  MEDLINE    
The induction of murine erythroleukemia cells (MELC) to terminal differentiation by hexamethylene bisacetamide (HMBA) is accompanied by changes in the levels of c-myb and c-myc mRNA, and in p53 protein levels. We simultaneously examined the effects of HMBA on modulation of c-myb, c-myc and p53 mRNA and protein levels, and examined the relationship between these changes and commitment to terminal cell division. In MELC cultured with HMBA, c-myb protein levels paralleled c-myb mRNA levels except at 24h, when the protein level was equivalent to the level in control cultures, whereas the mRNA had decreased. The c-myc protein paralleled c-myc mRNA throughout induction. The p53 mRNA and protein behaved in a discordant fashion. The p53 protein decreased to very low levels between 4 and 8 h and remained low, while the mRNA, which initially decreased, reaccumulated by 24 and 48 h. Transfer of MELC after 12 to 48 h of culture with HMBA to medium without inducer resulted in rapid (less than 3 h) reaccumulation of the c-myb mRNA, c-myb protein, and p53 protein, and cessation of recruitment of cells to commitment. Cells already induced to commit to terminal differentiation continued to express the differentiated phenotype.
V M Richon; R G Ramsay; R A Rifkind; P A Marks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  4     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-04-26     Completed Date:  1989-04-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  165-73     Citation Subset:  IM    
The DeWitt Wallace Research Laboratory, Memorial Sloan-Kettering Cancer Center, Cornell University, New York, NY 10021.
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MeSH Terms
Acetamides / pharmacology
Cell Differentiation / drug effects
Dexamethasone / pharmacology
Leukemia, Erythroblastic, Acute / metabolism*,  pathology
Neoplasm Proteins / analysis*,  genetics
Phosphoproteins / analysis*,  genetics
Proto-Oncogene Proteins / analysis*,  genetics
Proto-Oncogene Proteins c-myb
Proto-Oncogene Proteins c-myc
RNA, Messenger / analysis*
Tumor Suppressor Protein p53
Grant Support
Reg. No./Substance:
0/Acetamides; 0/Neoplasm Proteins; 0/Phosphoproteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; 3073-59-4/hexamethylene bisacetamide; 50-02-2/Dexamethasone

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