Document Detail


Modulation by retinyl acetate of microfilament bundle formation in C3H/10T1/2 cells.
MedLine Citation:
PMID:  6539338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retinyl acetate has been previously shown to inhibit carcinogen-induced neoplastic transformation in 10T1/2 cells and to accentuate many aspects of the nontransformed phenotype. Scanning electron microscopy of logarithmic phase 10T1/2 cells treated for 3 days with 0.3 micrograms/ml retinyl acetate revealed that this treatment caused extensive flattening of cells to the plastic substrate. In contrast the tumor promoter tetradecanoyl phorbol acetate, which antagonizes the antineoplastic activity of retinyl acetate, caused cell rounding and completely inhibited the action of retinyl acetate on cell morphology. During this same time course, the formation of microfilament bundles was also found to be modulated by retinyl acetate. Transmission electron micrographs of unsectioned peripheral regions of flattened cells showed that while the unit density of microfilament bundles was not influenced, the thickness of bundles, particularly those with a diameter of 100 nm or more, was increased by retinyl acetate. Tetradecanoyl phorbol acetate had little effect on microfilament bundle diameters but did partially antagonize the action of retinyl acetate. To determine if this increase was associated with an increase in total actin/cell, total cell proteins, and proteins not extractable by glycerol-triton extraction, were subjected to sodium dodecylsulfate/ polyacrylamide gel electro-phoresis. It was found that while total cellular actin was not increased by retinyl acetate, the proportion of nonextractable actin (which includes microfilament bundles) increased from 65% to 88% of total actin. This increase was not inhibited by inhibitors of protein or RNA synthesis. These studies again demonstrate that retinyl acetate accentuates the nontransformed phenotype of 10T1/2 cells; it is hypothesized that these actions are related to the antineoplastic activity of retinoids.
Authors:
L J Mordan; S W Hui; J S Bertram
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  24     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-10     Completed Date:  1984-07-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15-25     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cell Adhesion / drug effects
Cell Line
Cell Transformation, Neoplastic / drug effects
Cytoskeleton / drug effects*,  metabolism,  ultrastructure
Mice
Microscopy, Electron
Tetradecanoylphorbol Acetate / pharmacology
Vitamin A / analogs & derivatives*,  pharmacology
Grant Support
ID/Acronym/Agency:
CA 25484/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 11103-57-4/Vitamin A; 127-47-9/retinol acetate; 16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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