| Modulation of autophagy and ubiquitin-proteasome pathways during ultra-endurance running. | |
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MedLine Citation:
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PMID: 22345427 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study, the coordinated activation of ubiquitin-proteasome pathways (UPP), autophagy-lysosomal (ALP) and mitochondrial remodelling including mitophagy was assessed by measuring protein markers during ultra-endurance running exercise in human skeletal muscle. Eleven male experienced ultra-endurance athletes ran for 24 hours on a treadmill. Muscle biopsy samples were taken from the vastus lateralis muscle two hours before starting and immediately after finishing exercise. Athletes ran 149.8 ± 16.3 km with an effective running time of 18h 42min (± 41min). The phosphorylation state of Akt (-74 ± 5 %, P < 0.001), FoxO3a (-49 ± 9 %, P < 0.001), mTOR Ser2448 (-32 ± 14 %, P = 0.028) and 4E-BP1 (-34 ± 7 %, P < 0.001) was decreased whereas AMPK phosphorylation state increased by 247 ± 170 % (P = 0.042). Proteasome β2 subunit activity increased by 95 ± 44 % (P = 0.028) while the activities associated to the β1 and β5 subunits remained unchanged. MuRF1 protein level increased by 55 ± 26 % (P = 0.034) while MAFbx protein and ubiquitin-conjugated protein levels did not change. LC3bII increased by 554 ± 256% (P = 0.005) and the form of ATG12 conjugated to ATG5 increased by 36 ± 17% (P = 0.042). The mitochondrial fission marker phospho-DRP1 increased by 110 ± 47% (P = 0.003) whereas the fusion marker Mfn1 and the mitophagy markers Parkin and PINK1 remained unchanged. These results fit well with a coordinated regulation of ALP and UPP triggered by FoxO3 and AMPK during ultra-endurance exercise. |
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Authors:
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Cécile Jamart; Marc Francaux; Guillaume Y Millet; Louise Deldicque; Delphine Frere; Léonard Féasson |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-16 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-2-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1UCLouvain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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