Document Detail

Modulation of the antioxidant activities in dox-sensitive and -resistant Friend leukemia cells. Effect of doxorubicin.
MedLine Citation:
PMID:  2064348     Owner:  NLM     Status:  MEDLINE    
Tumor cell resistance to anthracyclines has been associated with increased activity against free radicals. Here, we have investigated the direct effect of doxorubicin (DOX) in the modulation of glutathione level and antioxidant activities in DOX-sensitive and-resistant cells (288 fold). The glutathione level in untreated cells was 88% greater in resistant than in sensitive cells. The activities of the superoxide dismutase, glutathione -S-transferase and glutathione reductase were respectively 24, 15 and 38% higher in resistant cells than in their sensitive counterparts. In contrast, catalase and total glutathione peroxidase were reduced in resistant cells by 18 and 21% respectively. Moreover, the activity of selenium-dependent glutathione peroxidase was lowered by 47% in the resistant as compared to the sensitive cells. Exposure of sensitive or resistant cells to low doses of DOX did not affect these levels in either cell variant. It is concluded therefore that resistance to anthracyclines may not always be associated with an elevated level of intracellular antioxidant activity enzymes.
M Crescimanno; N D'Alessandro; M G Armata; S Toulmond; H Tapiero
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  11     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1991 Mar-Apr
Date Detail:
Created Date:  1991-08-06     Completed Date:  1991-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  901-3     Citation Subset:  IM    
Istituto di Farmacologia, Policinico P. Giaccone, Palermo, Italy.
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MeSH Terms
Antioxidants / metabolism*
Catalase / metabolism
Doxorubicin / pharmacology*
Drug Resistance / physiology
Friend murine leukemia virus
Glutathione / metabolism*
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Glutathione Transferase / metabolism
Isoenzymes / metabolism
Leukemia, Experimental / enzymology*
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Isoenzymes; 23214-92-8/Doxorubicin; 70-18-8/Glutathione; EC; EC Peroxidase; EC Dismutase; EC Reductase; EC Transferase

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