Document Detail


Modulation of acetylcholine-induced secretion of gastric bombesin-like immunoreactivity by cholinergic and histamine H2-receptors, somatostatin and intragastric pH.
MedLine Citation:
PMID:  6146996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently we have shown the release of bombesin-like immunoreactivity (BLI) from the isolated perfused rat stomach. In these experiments we have shown that BLI secretion is stimulated by acetylcholine. Gastric inhibitory peptide (GIP) exerts an inhibitory effect which is dependent on the intraluminal pH. The present study was designed to examine further the exact cholinergic mechanisms and to study the interaction between cholinergic and histaminergic mechanisms as well as the effect of the intraluminal pH. Acetylcholine elicited a dose-dependent increase in BLI and gastrin secretion (10(-6) M and 2 X 10(-6)M), whereas somatostatin release was suppressed at luminal pH 7. Blockade of muscarinic cholinergic receptors by atropine (10(-5)M) and nicotinic cholinergic receptors by hexamethonium (10(-5) M) abolished the effect of acetylcholine on all three peptides. Reduction of the intraluminal pH to 2 also abolished acetylcholine-induced stimulation of BLI and gastrin secretion and the inhibition of somatostatin secretion. Changes of intraluminal pH per se had no effect on the secretion of either peptide. Somatostatin (10(-7) M) reduced both BLI and gastrin secretion during stimulation with acetylcholine. The addition of the H2-receptor antagonist cimetidine (10(-5) M) abolished the effect of both doses of acetylcholine on BLI and somatostatin secretion and also the effect of the lower dose of acetylcholine (10(-6) M) on gastrin secretion during luminal pH 7. At luminal pH 2 cimetidine did not alter BLI and somatostatin secretion in response to acetylcholine, however, gastrin release was augmented in the presence of cimetidine. These data demonstrate that the effect of acetylcholine on BLI, gastrin, and somatostatin secretion is mediated by muscarinic and nicotinic cholinergic receptors and also by histamine H2-receptors. Somatostatin inhibits cholinergically induced BLI secretion. The cholinergic effects on BLI, somatostatin and gastrin secretion are abolished during an acidic intragastric pH. In this isolated perfused rat stomach model the inhibitory effect of intraluminal acid on gastrin secretion is, at least in part, mediated by H2-receptors. This suggests that the secretion of bombesin, a potential peptidergic neurotransmitter is modulated by neural, endocrine and local tissue factors and also by alterations of intragastric pH.
Authors:
V Schusdziarra; H Bender; A Pfeffer; E F Pfeiffer
Related Documents :
25394486 - Oligomeric abeta-induced synaptic dysfunction in alzheimer's disease.
10398816 - Tumour uptake of the radiolabelled somatostatin analogue [dota0, tyr3]octreotide is dep...
6126856 - Characterization of the vip- and secretin-stimulated adenylate cyclase system from huma...
15587336 - Endocrine tumours, somatostatin and somatostatin receptors.
1347006 - Dopamine stimulates growth hormone release from the pituitary of goldfish, carassius au...
19318116 - Cholinergic terminals in the ventral horn of adult rat and cat: evidence that glutamate...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Regulatory peptides     Volume:  8     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-08-24     Completed Date:  1984-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  189-98     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology*
Animals
Atropine / pharmacology
Bombesin / secretion*
Cimetidine / pharmacology
Gastric Acid / physiology*
Gastric Mucosa / secretion*
Gastrins / secretion
Hexamethonium
Hexamethonium Compounds / pharmacology
Kinetics
Peptides / secretion*
Radioimmunoassay / methods
Rats
Receptors, Cholinergic / physiology*
Receptors, Histamine / physiology*
Receptors, Histamine H2 / physiology*
Somatostatin / pharmacology,  secretion*
Chemical
Reg. No./Substance:
0/Gastrins; 0/Hexamethonium Compounds; 0/Peptides; 0/Receptors, Cholinergic; 0/Receptors, Histamine; 0/Receptors, Histamine H2; 31362-50-2/Bombesin; 51-55-8/Atropine; 51-84-3/Acetylcholine; 51110-01-1/Somatostatin; 51481-61-9/Cimetidine; 60-26-4/Hexamethonium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Blood and plasma kinetics of cis(Z)-clopenthixol and fluphenazine in psychiatric patients after intr...
Next Document:  Increases in arterial blood pressure in the rat in response to a new vertebrate neuropeptide, LPLRFa...