Document Detail

Modulation of the absorption maximum of rhodopsin by amino acids in the C-terminus.
MedLine Citation:
PMID:  16922606     Owner:  NLM     Status:  MEDLINE    
Vision begins when light is absorbed by visual pigments. It is commonly believed that the absorption spectra of visual pigments are modulated by interactions between the retinal and amino acids within or near 4.5 angstroms of the retinal in the transmembrane (TM) segments. However, this dogma has not been rigorously tested. In this study, we show that the retinal-opsin interactions extend well beyond the retinal binding pocket. We found that, although it is positioned outside of TM segments, the C-terminus of the rhodopsin in the rockfish longspine thornyhead (Sebastolobus altivelis) modulates its lambda(max) by interacting mainly with the last TM segment. Our results illustrate how amino acids in the C-terminus are likely to interact with the retinal. We anticipate our analyses to be a starting point for viewing the spectral tuning of visual pigments as interactions between the retinal and key amino acids that are distributed throughout the entire pigment.
Shozo Yokoyama; Takashi Tada; Takahisa Yamato
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  83     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:    2007 Mar-Apr
Date Detail:
Created Date:  2007-09-05     Completed Date:  2007-10-19     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  236-41     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Amino Acids / chemistry
Base Sequence
COS Cells
Cercopithecus aethiops
DNA Primers / genetics
Fishes / genetics
Models, Molecular
Molecular Sequence Data
Recombinant Proteins / chemistry,  genetics
Rhodopsin / chemistry*,  genetics
Sequence Homology, Amino Acid
Species Specificity
Grant Support
R01 EY016400/EY/NEI NIH HHS; R01 EY016400-02/EY/NEI NIH HHS
Reg. No./Substance:
0/Amino Acids; 0/DNA Primers; 0/Recombinant Proteins; 9009-81-8/Rhodopsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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