| Modulation of Thyroid Hormone Concentrations in Serum of Rats Coadministered with Perchlorate and Iodide-Deficient Diet. | |
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MedLine Citation:
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PMID: 21573761 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Perchlorate can perturb thyroid hormone (TH) homeostasis by competitive inhibition of iodide uptake by the thyroid gland. Until recently, the effects of perchlorate on TH homeostasis were examined by measuring serum concentrations of THs by immunoassay (IA) methods. IA methods are sensitive, but for TH analysis they are compromised by lack of adequate specificity. In this study, we determined the concentrations of six THs: L: -thyroxine (T(4)), 3,3',5-triiodo-L: -thyronine (T(3)), 3,3',5'-triiodo-L: -thyronine (rT(3)), 3,5-diiodo-L: -thyronine, 3,3'-diiodo-L: -thyronine, and 3-iodo-L: -thyronine in the serum of rats administered perchlorate by isotope ((13)C(6)-T(4))-dilution liquid chromatography-tandem mass spectrometry. The method recoveries for THs spiked into a serum matrix were between 97.0% and 115%, with a coefficient of variation of 2.1% to 9.4%. Rats were placed on an iodide-deficient or iodide-sufficient diet for 2.5 months, and for the last 2 weeks of that period they were provided drinking water either without or with perchlorate (10 mg/kg body weight/day). No significant differences in serum concentrations of T(3) and T(4) were observed between rats given iodide-deficient and iodide-sufficient diets for 2 or 2.5 months. After 24 h of perchlorate exposure, significantly lower concentrations of T(3) and T(4) were found in the serum of rats administered the iodide-deficient diet but not in rats administered the iodide-sufficient diet. However, after 2 weeks of perchlorate exposure, TH levels in rats fed the iodide-sufficient diet were also significantly lower than those in control rats. Our results suggest that perchlorate affects TH homeostasis and that such effects are more pronounced under iodide-deficient nutrition. |
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Authors:
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Tatsuya Kunisue; Jeffrey W Fisher; Kurunthachalam Kannan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-15 |
Journal Detail:
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Title: Archives of environmental contamination and toxicology Volume: - ISSN: 1432-0703 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0357245 Medline TA: Arch Environ Contam Toxicol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Wadsworth Center, New York State Department of Health, Empire State Plaza, P.O. Box 509, Albany, NY, 12201-0509, USA. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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