Document Detail


Modulation of mammalian cell processes by bacterial quorum sensing molecules.
MedLine Citation:
PMID:  21031309     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Microbial pathogens use a wide repertoire of pathogen-associated molecular patterns (PAMPs) that affect host cell responses through activation of intracellular signaling events in a PAMP-specific manner. Here we describe a set of western blot-based methodologies for the evaluation of biochemical effects specifically induced by N-(3-oxo-acyl) homoserine lactones (3-oxo-AHLs) small molecules secreted by a number of Gram-negative bacteria, including the opportunistic human pathogen Pseudomonas aeruginosa. First, we will highlight the AHL-mediated effects on proapoptotic and stress pathways. Secondly, we will demonstrate that AHLs possess the ability to alter stimulus-induced NF-κB signaling, a key biochemical marker of inflammation and innate immune responses.
Authors:
Vladimir V Kravchenko; Richard J Ulevitch; Gunnar F Kaufmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  692     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-10-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-45     Citation Subset:  IM    
Affiliation:
Department of Immunology & Microbial Science, The Scripps Research Institute, La Jolla, CA, USA.
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
AI079436/AI/NIAID NIH HHS; AI080715/AI/NIAID NIH HHS; R01 AI080715-03/AI/NIAID NIH HHS

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