Document Detail


Modulation of IK,Ca by phorbol ester-mediated activation of PKC in pleural sensory neurons of Aplysia.
MedLine Citation:
PMID:  1432069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The electrophysiological properties of the sensory neurons that mediate withdrawal reflexes in Aplysia are modulated by a number of second messengers. For example, the second messengers adenosine 3',5'-cyclic monophosphate (cAMP) and arachidonic acid modulate the S-K+ current (IK,S) and the calcium-activated K+ current (IK,Ca). Recent evidence suggests that protein kinase C (PKC) may also be an important regulator of cellular plasticity. In the present study we examined the possibility that IK,Ca was modulated by the activation of PKC in the pleural sensory neurons. 2. In voltage-clamped sensory neurons the application of phorbol esters, such as phorbol dibutyrate (PDBu), phorbol myristate (PMA), and phorbol diacetate (PDAc), which activate PKC, caused a dose-dependent increase in a voltage-dependent current with properties that resembled IK,Ca. The inactive isomer of phorbol ester, 4 alpha-phorbol, was without effect. 3. This phorbol ester-sensitive current had the kinetics and pharmacological sensitivity of IK,Ca. The current developed slowly during step depolarizations, showed little inactivation, and was activated at membrane potentials greater than approximately 0 mV. In addition, the current modulated by phorbol esters was blocked by a concentration of tetraethylammonium (TEA) that blocks a component of IK,Ca in the sensory neurons. 4. IK,Ca, which was activated directly by the iontophoretic injection of Ca2+, was also enhanced by PDBu. Moreover, the enhancement of Ca(2+)-elicited responses by PDBu persisted after Ca2+ influx was blocked by cobalt. These results indicate that at least one component of the modulation of IK,Ca by PDBu was independent of the modulation of voltage-dependent Ca2+ channels.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
S D Critz; J H Byrne
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  68     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-17     Completed Date:  1992-12-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1079-86     Citation Subset:  IM    
Affiliation:
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225.
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MeSH Terms
Descriptor/Qualifier:
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Animals
Aplysia
Calcium / pharmacology
Dose-Response Relationship, Drug
Enzyme Activation
Ganglia / physiology
Isoquinolines / pharmacology
Kinetics
Membrane Potentials / drug effects
Neurons, Afferent / drug effects,  enzymology,  physiology*
Phorbol 12,13-Dibutyrate / pharmacology*
Phorbol Esters / pharmacology*
Phorbols / pharmacology
Piperazines / pharmacology
Potassium Channels / drug effects,  physiology*
Protein Kinase C / metabolism*
Protein Kinase Inhibitors
Second Messenger Systems
Tetradecanoylphorbol Acetate / pharmacology*
Tetraethylammonium
Tetraethylammonium Compounds / pharmacology
Grant Support
ID/Acronym/Agency:
F32-NS08579/NS/NINDS NIH HHS; K02-MH00649/MH/NIMH NIH HHS; NS-19895/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Isoquinolines; 0/Phorbol Esters; 0/Phorbols; 0/Piperazines; 0/Potassium Channels; 0/Protein Kinase Inhibitors; 0/Tetraethylammonium Compounds; 16561-29-8/Tetradecanoylphorbol Acetate; 17673-25-5/phorbol; 24928-17-4/phorbol-12,13-didecanoate; 37558-16-0/Phorbol 12,13-Dibutyrate; 66-40-0/Tetraethylammonium; 7440-70-2/Calcium; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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