| Modulation of human serum glutathione S-transferase A1/2 concentration by cruciferous vegetables in a controlled feeding study is influenced by GSTM1 and GSTT1 genotypes. | |
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MedLine Citation:
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PMID: 19900941 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutathione S-transferases (GST) detoxify a wide range of carcinogens. Isothiocyanates (ITC), from cruciferous vegetables, are substrates for and inducers of GST. GST variants may alter ITC clearance such that response to crucifers varies by genotype. In a randomized cross-over trial, we tested the hypothesis that changes in serum GSTA1/2 concentration in response to cruciferous vegetable feeding depends on GSTM1/GSTT1 genotype. Thirty-three men and 34 women (age 20-40 years) ate four 14-day controlled diets--basal (vegetable-free), basal supplemented with two different doses of crucifers ("single dose" and "double dose"), and single-dose cruciferous-plus-apiaceous vegetables--fed per kilogram of body weight. Fasting bloods from days 0, 7, 11, and 14 of each diet period were analyzed for serum GSTA1/2 by ELISA. GSTA1/2 increased with single- and double-dose cruciferous compared with basal diet (10% and 13%, respectively; P = 0.02 and 0.004), but cruciferous-plus-apiaceous did not differ from basal (P = 0.59). Overall, GSTA1/2 was higher in GSTM1-null/GSTT1-null than GSTM1+/GSTT1+ individuals (4,198 +/- 338 and 3,372 +/- 183 pg/mL; P = 0.03). The formal interaction of genotype-by-diet was not statistically significant, but the GSTA1/2 increase during the single-dose cruciferous diet was among GSTM1-null/GSTT1-null individuals (by 28%; P = 0.008), largely explained by GSTM1-null/GSTT1-null men (by 41%; P = 0.01). GSTA1/2 increased during the double-dose cruciferous diet in both GSTM1-null/GSTT1-null men (by 35%; P = 0.04) and GSTM1+/GSTT1+ men (by 26%; P = 0.01) but not in women. In summary, cruciferous vegetable supplementation increased GSTA1/2, but the effect was most marked in GSTM1-null/GSTT1-null men. |
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Authors:
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Sandi L Navarro; Jyh-Lurn Chang; Sabrina Peterson; Chu Chen; Irena B King; Yvonne Schwarz; Shuying S Li; Lin Li; John D Potter; Johanna W Lampe |
Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology Volume: 18 ISSN: 1538-7755 ISO Abbreviation: Cancer Epidemiol. Biomarkers Prev. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-10 Completed Date: 2010-01-27 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 9200608 Medline TA: Cancer Epidemiol Biomarkers Prev Country: United States |
Other Details:
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Languages: eng Pagination: 2974-8 Citation Subset: IM |
Affiliation:
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Cancer Prevention Program, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cross-Over Studies Female Genotype Glutathione Transferase / blood*, genetics* Homozygote Humans Isoenzymes / blood* Male Phytotherapy* Prognosis Tumor Markers, Biological / genetics* Vegetables* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA070913-08/CA/NCI NIH HHS; R01CA070913/CA/NCI NIH HHS; R25 CA094880-10/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/Tumor Markers, Biological; EC 2.5.1.-/glutathione S-transferase T1; EC 2.5.1.18/GSTA1 protein, human; EC 2.5.1.18/Glutathione Transferase; EC 2.5.1.18/glutathione S-transferase M1; EC 2.5.1.18/glutathione S-transferase alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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