Document Detail


Modulation of Epstein-Barr virus latent membrane protein 1 activity by intrabodies.
MedLine Citation:
PMID:  17460414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The Epstein-Barr virus (EBV) has been implicated in the development of many human neoplasias including B lymphoma and nasopharyngeal carcinoma. EBV latent membrane protein 1 (LMP1) is essential to virus-induced B cell immortalization and the downregulation of cell adhesion molecules that increases cell motility. Therefore, identifying LMP1 activity modulation methods may lead to the development of new therapies for LMP1-positive tumors. METHODS: This study uses a phage display single-chain variable fragments (scFvs) library to screen recombinant antibodies specific to the LMP1 C terminal region. A total of 45 individual clones were obtained, and these scFvs were cloned as intrabodies and transfected into LMP1-positive cells. RESULTS: One of the scFv clones, designated H3, was capable of reducing LMP1-mediated NF-kappaB activation in HEK293 cells. Immunofluorescence and co-immunoprecipitation studies show that scFv H3 could interact with LMP1 in vivo. In addition, expression of scFv H3 intrabody could reduce cell motility in MDCK-LMP1 cells in the transwell migration assay. CONCLUSION: These data indicate that scFv H3 intrabody can inhibit LMP1 functions in epithelial cells and may be useful for attenuating the LMP1 function in LMP1-positive tumors.
Authors:
Chih Yeu Fang; Chen Yi Chiang; Yun Ru Pan; Ka Po Tse; Yu Sun Chang; Hwan You Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-25
Journal Detail:
Title:  Intervirology     Volume:  50     ISSN:  1423-0100     ISO Abbreviation:  Intervirology     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-23     Completed Date:  2007-10-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364265     Medline TA:  Intervirology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  254-63     Citation Subset:  IM    
Copyright Information:
Copyright 2007 S. Karger AG, Basel.
Affiliation:
Institute of Molecular Medicine, National Tsing Hua University, Hsin-Chu, Taiwan, ROC.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ201310;  DQ201311;  DQ201312;  DQ201313;  DQ201314;  DQ201315
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Viral / chemistry,  genetics,  immunology*
Antibody Specificity*
Cell Line
Cell Line, Tumor
Cell Movement / immunology
Dogs
Epithelial Cells
Herpesvirus 4, Human / pathogenicity*
Humans
Immunoglobulin Variable Region / chemistry,  genetics,  immunology*
Molecular Sequence Data
Peptide Library
Recombinant Proteins / chemistry,  genetics,  immunology
Sequence Analysis, DNA
Transfection
Viral Matrix Proteins / chemistry,  immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/EBV-associated membrane antigen, Epstein-Barr virus; 0/Immunoglobulin Variable Region; 0/Peptide Library; 0/Recombinant Proteins; 0/Viral Matrix Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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