Document Detail


Modulation of Cholesterol Transport by Insulin-Treated Gestational Diabetes Mellitus in Human Full Term Placenta.
MedLine Citation:
PMID:  23221398     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Gestational diabetes mellitus (GDM) is a common complication of pregnancy which is characterized by glucose intolerance, leads to dyslipidemia and is aggravated by obesity. Cholesterol is taken up by the placenta as part of lipoproteins through the receptors SRBI, LDLR, VLDLR and its efflux is then mediated by ABCA1 and ABCG1. PCSK9 is involved in the degradation of LDLR and VLDLR. The goal of this study was to evaluate the impact of GDM and pre-pregnancy body mass index (BMI) on cholesterol transport through the modulation of the expression of several key players. Human full-term placenta, maternal and venous cord blood samples were obtained at delivery from normal weight women without GDM (n = 10), normal weight women with GDM (n = 6) and overweight/obese women with GDM (n = 6). Lipids (total cholesterol, HDL, LDL, triglycerides, free fatty acids, apolipoprotein A1, apolipoprotein B100) levels were evaluated in blood samples. Messenger RNA and protein expression levels (LDLR, VLDLR, SRBI, ABCA1, ABCG1, PCSK9, LXRs, PPARs) were assessed in human full-term placenta respectively by real-time RT-PCR and western blots. LPL activity was evaluated using a commercial kit on tissue homogenates. Overall, our study demonstrates that GDM affects the maternal and neonatal lipid profiles as well as different key players of placental cholesterol transfer from the maternal to the fetal circulation depending on the maternal BMI. These changes could affect the fetal metabolism and predispose the fetus to future metabolic diseases.
Authors:
Evemie Dubé; Maude Ethier-Chiasson; Julie Lafond
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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