Document Detail

Modulation of bacterial ghosts--induced nitric oxide production in macrophages by bacterial ghost-delivered resveratrol.
MedLine Citation:
PMID:  23289719     Owner:  NLM     Status:  MEDLINE    
The present study aimed to investigate the capacity of resveratrol (RV) delivered into macrophages by bacterial ghosts (BGs), representing intact empty nonliving envelopes of Gram-negative bacteria, to modulate nitric oxide (NO) production related to the presence of the pathogen-associated molecular patterns on the surface of BGs. Incubation of the murine macrophage cell line RAW 264.7 with BGs leads to a dose-dependent activation of inducible NO synthase. To modify BG-induced NO formation in RAW 264.7 cells by RV; BGs were loaded with RV (RV-BGs) and incubated with murine macrophages in a dose-dependent manner. RV-BGs delivering RV to the target macrophages significantly reduced BG-induced NO production with concentration of RV more than one order of magnitude lower than the amount of RV capable of reducing NO formation when applied directly. Moreover, no cytotoxic impact of BGs on the viability of RAW 264.7 cells added to macrophages alone or loaded with RV was detected after a mutual 24 h incubation, whereas cell viability slightly decreased (~ 10%) when RV concentrations of 30 μm alone were applied. The results obtained in the present study clearly indicate that the intracellular delivery of RV by BGs significantly enhances the total RV effect.
Verena J Koller; Verena M Dirsch; Hortenzia Beres; Oliver Donath; Gottfried Reznicek; Werner Lubitz; Pavol Kudela
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-31
Journal Detail:
Title:  The FEBS journal     Volume:  280     ISSN:  1742-4658     ISO Abbreviation:  FEBS J.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-04-18     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  101229646     Medline TA:  FEBS J     Country:  England    
Other Details:
Languages:  eng     Pagination:  1214-25     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Journal compilation © 2012 FEBS.
Department of Medicinal Chemistry, University of Vienna, Vienna, Austria.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Antigen-Presenting Cells / immunology
Cell Membrane / drug effects,  metabolism
Cell Survival
Cells, Cultured
Cytoplasm / metabolism
Drug Delivery Systems*
Escherichia coli / immunology*
Macrophages / drug effects,  immunology,  metabolism*
Nitric Oxide / metabolism*
Nitric Oxide Synthase Type II / metabolism
Stilbenes / pharmacology*
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Stilbenes; 10102-43-9/Nitric Oxide; EC Oxide Synthase Type II; Q369O8926L/resveratrol

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