| Modulation of adipocyte biology by δ(9)-tetrahydrocannabinol. | |
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MedLine Citation:
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PMID: 20467421 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is recognized that the endocannabinoid system (ECS) plays a crucial role in the modulation of food intake and other aspects of energy metabolism. In this study, we aimed to investigate the effects of Δ(9)-tetrahydrocannabinol (THC) on adipocyte biology. 3T3-L1 cells were used to evaluate proliferation by sulforhodamine B (SRB) staining and methyl-(3)H-thymidine incorporation after 48 or 72 h of treatment with THC (1-500 nmol/l). Cells were differentiated in the presence or absence of the cannabinoid, and adipogenesis was determined by measuring lipid accumulation and peroxisome proliferator-activated receptor γ (PPARγ) transcription through reverse transcriptase-PCR (RT-PCR). Lipolysis was quantified under basal conditions or after isoproterenol (IP, 100 nmol/l) or insulin (INS, 100 nmol/l) treatment. Transforming growth factor β (TGFβ), diacylglycerol lipase α, and N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD) transcriptions were determined by RT-PCR in preadipocytes and adipocytes and adiponectin only in adipocytes. THC treatment increased culture protein content and reduced methyl-(3)H-thymidine incorporation. Cells treated with THC underwent adipogenesis shown by the expression of PPARγ and had increased lipid accumulation. Basal and IP-stimulated lipolyses were inhibited by THC and there was no effect on lipolysis of INS-treated adipocytes. The effects on methyl-(3)H-thymidine incorporation and lipolysis seem to be mediated through CB1- and CB2-dependent pathways. THC decreased NAPE-PLD in preadipocytes and increased adiponectin and TGFβ transcription in adipocytes. These results show that the ECS interferes with adipocyte biology and may contribute to adipose tissue (AT) remodeling. Although these observations point toward increased AT deposition, the stimulation of adiponectin production and inhibition of lipolysis may be in favor of improved INS sensitivity under cannabinoid influence. |
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Authors:
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Diana Teixeira; Diogo Pestana; Ana Faria; Conceição Calhau; Isabel Azevedo; Rosário Monteiro |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-13 |
Journal Detail:
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Title: Obesity (Silver Spring, Md.) Volume: 18 ISSN: 1930-739X ISO Abbreviation: Obesity (Silver Spring) Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-27 Completed Date: 2011-04-14 Revised Date: 2012-08-13 |
Medline Journal Info:
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Nlm Unique ID: 101264860 Medline TA: Obesity (Silver Spring) Country: United States |
Other Details:
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Languages: eng Pagination: 2077-85 Citation Subset: IM |
Affiliation:
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Department of Biochemistry U38-FCT, Faculty of Medicine, University of Porto, Porto, Portugal. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Adipocytes / cytology, drug effects*, metabolism Adipogenesis / drug effects*, physiology Adiponectin / metabolism Animals Cannabis / chemistry Insulin / pharmacology Insulin Resistance* Isoproterenol / pharmacology Lipid Metabolism / drug effects* Lipolysis / drug effects Lipoprotein Lipase / metabolism Mice PPAR gamma / metabolism Phospholipase D / metabolism Proteins / metabolism Psychotropic Drugs / pharmacology* Receptors, Cannabinoid / metabolism* Reverse Transcriptase Polymerase Chain Reaction Tetrahydrocannabinol / pharmacology* Transforming Growth Factor beta / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Insulin; 0/PPAR gamma; 0/Proteins; 0/Psychotropic Drugs; 0/Receptors, Cannabinoid; 0/Transforming Growth Factor beta; 1972-08-3/Tetrahydrocannabinol; 7683-59-2/Isoproterenol; EC 3.1.1.34/Lipoprotein Lipase; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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