| Modulation of 8-methoxypsoralen-DNA photoadduct formation by cell differentiation, mitogenic stimulation and phorbol ester exposure in murine T lymphocytes. | |
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MedLine Citation:
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PMID: 8310003 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of cell differentiation and mitogen and phorbol ester stimulation on the formation of 8-methoxypsoralen (8-MOP)-DNA photoadducts in murine T lymphocytes were examined using 3H-8-MOP. While there were no significant differences in 8-MOP photoadduct formation among BALB/c thymocytes, splenocytes, splenic T cells and MRL/lpr lymph node cells, BALB/c bone marrow cells showed fewer photoadducts than did the lymphocytes. This suggested that proliferating progenitor cells may be resistant to 8-MOP photoadduct formation. Incubation of purified splenic T cells with lectin mitogens for 2 h or with phorbol 12-myristate 13-acetate (PMA) for 2-43 h resulted in reduction of 8-MOP photoadduct formation in the DNA, whereas 64 h cultivation with these agents augmented the photoadduct formation. The reduction of photoadduct formation induced by phytohemagglutinin was restored by the further addition of a protein kinase C (PKC) inhibitor, H-7, to the culture. Thus, it is assumed that the reduction of adduct formation evoked by mitogens and PMA is mediated in part by the activation of PKC in the cells. On the other hand, the augmentation of the adduct formation induced by the longer-period cultures with mitogens and PMA appeared to be caused by down-regulation of PKC. The present study showed that the stimulatory signals in which PKC is presumably involved affect the ability of cells to form 8-MOP-DNA photoadducts. |
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Authors:
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Y Tokura; R L Edelson; F P Gasparro |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 58 ISSN: 0031-8655 ISO Abbreviation: Photochem. Photobiol. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-03-16 Completed Date: 1994-03-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 822-6 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Hamamatsu University School of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Cells Cell Differentiation* Cells, Cultured DNA Damage / drug effects* Female Methoxsalen / pharmacology* Mice Mice, Inbred BALB C Mice, Inbred Strains Mitogens / pharmacology* Spleen / cytology T-Lymphocytes / drug effects* Tetradecanoylphorbol Acetate / pharmacology* Thymus Gland / cytology Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
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0/Mitogens; 16561-29-8/Tetradecanoylphorbol Acetate; 298-81-7/Methoxsalen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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