| Modulating signaling events in smooth muscle: cleavage of annexin 2 abolishes its binding to lipid rafts. | |
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MedLine Citation:
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PMID: 12153985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell membrane compartmentalization, which is believed to involve association of cholesterol- and glycosphingolipid-enriched membrane rafts, represents an important means of transmitting information across the plasma membrane. We have previously shown that raft association is mediated by the Ca2+-dependent binding of annexin 2 to the plasma membrane. In the present study, we demonstrate that the association of annexins 1 and 2 with the smooth muscle cell membrane can be terminated by their proteolytic cleavage. This proteolysis is thought to be triggered by calpain and occurs at non-raft regions of the plasma membrane. It is critically dependent on the intracellular concentration of free Ca2+ and requires an intact contractile apparatus. Annexins 1 and 2 interact with different membrane microcompartments--the former with non-raft, glycerolipid regions, the latter preferentially with membrane rafts. We demonstrate that PKC and RhoA, major signaling molecules that regulate smooth muscle contraction, are spatially segregated and interact with distinct membrane microcompartments. Proteolysis abolishes annexin binding to the plasma membrane and might result in rearrangement of membrane constituents followed by the interruption of segregation-dependent signaling events. |
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Authors:
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Eduard B Babiychuk; Katia Monastyrskaya; Fiona C Burkhard; Susan Wray; Annette Draeger |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 16 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-02 Completed Date: 2002-09-03 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 1177-84 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Institute of Anatomy, University of Bern, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5'-Nucleotidase
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analysis Animals Annexin A1 / metabolism Annexin A2 / metabolism* Annexins / analysis Biological Markers / analysis Cells, Cultured Culture Techniques HeLa Cells Humans Intracellular Membranes / chemistry Membrane Microdomains / chemistry, metabolism* Models, Biological Muscle Contraction Muscle, Smooth / metabolism*, physiology Protein Kinase C / analysis Signal Transduction* Swine rhoA GTP-Binding Protein / analysis |
| Chemical | |
Reg. No./Substance:
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0/Annexin A1; 0/Annexin A2; 0/Annexins; 0/Biological Markers; EC 2.7.11.13/Protein Kinase C; EC 3.1.3.5/5'-Nucleotidase; EC 3.6.5.2/rhoA GTP-Binding Protein |
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