Document Detail


Modulating effect of resveratrol and quercetin on oral cancer cell growth and proliferation.
MedLine Citation:
PMID:  10211549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Resveratrol and quercetin are polyphenols which have been detected in significant amounts in green vegetables, citrus fruits and red grape wines. Beneficial effects attributed to these compounds include anti-inflammatory, antiviral and antitumor properties. The effect of resveratrol and quercetin on growth of human oral cancer cells is unknown. Resveratrol and quercetin, in concentrations of 1 to 100 microM, were incubated in triplicates with human oral squamous carcinoma cells SCC-25 in DMEM-HAM's F-12 supplemented with fetal calf serum and antibiotics in an atmosphere of 5% CO2 in air at 37 degrees C for 72 h. Cell growth was determined by counting the number of viable cells with a hemocytometer. Cell proliferation was measured by means of incorporation of [3H]thymidine in nuclear DNA. Resveratrol at 10 and 100 microM induced significant dose-dependent inhibition in cell growth as well as in DNA synthesis. Quercetin exhibited a biphasic effect, stimulation at 1 and 10 microM, and minimal inhibition at 100 microM in cell growth and DNA synthesis. Combining 50 microM of resveratrol with 10, 25 and 50 microM of quercetin resulted in a gradual and significant increase in the inhibitory effect of quercetin on cell growth and DNA synthesis. We conclude that resveratrol or a combination of resveratrol and quercetin, in concentrations equivalent to that present in red wines, are effective inhibitors of oral squamous carcinoma cell (SCC-25) growth and proliferation, and warrant further investigation as cancer chemopreventive agents.
Authors:
T M ElAttar; A S Virji
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Anti-cancer drugs     Volume:  10     ISSN:  0959-4973     ISO Abbreviation:  Anticancer Drugs     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-03     Completed Date:  1999-06-03     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9100823     Medline TA:  Anticancer Drugs     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  187-93     Citation Subset:  IM    
Affiliation:
Hormone Research Laboratory, University of Missouri-Kansas City, School of Dentistry, 64108-2784, USA. virjia@umkc.edu
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Antineoplastic Agents, Phytogenic / administration & dosage,  pharmacology*
Antineoplastic Combined Chemotherapy Protocols / pharmacology
Carcinoma, Squamous Cell / drug therapy*,  pathology
Cell Division / drug effects
Cisplatin / pharmacology
DNA, Neoplasm / drug effects
Dose-Response Relationship, Drug
Humans
Indomethacin / pharmacology
Quercetin / administration & dosage*
Stilbenes / administration & dosage*
Tongue Neoplasms / drug therapy*,  pathology
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antineoplastic Agents, Phytogenic; 0/DNA, Neoplasm; 0/Stilbenes; 117-39-5/Quercetin; 15663-27-1/Cisplatin; 53-86-1/Indomethacin; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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