Document Detail

Modulating bone cells response onto starch-based biomaterials by surface plasma treatment and protein adsorption.
MedLine Citation:
PMID:  17011619     Owner:  NLM     Status:  MEDLINE    
The effect of oxygen-based radio frequency glow discharge (rfGD) on the surface of different starch-based biomaterials (SBB) and the influence of proteins adsorption on modulating bone-cells behavior was studied. Bovine serum albumin, fibronectin and vitronectin were used in single and complex protein systems. RfGD-treated surfaces showed to increase in hydrophilicity and surface energy when compared to non-modified SBB. Biodegradable polymeric blends of cornstarch with cellulose acetate (SCA; 50/50wt%), ethylene vinyl alcohol (SEVA-C; 50/50wt%) and polycaprolactone (SPCL; 30/70wt%) were studied. SCA and SCA reinforced with 10% hydroxyapatite (HA) showed the highest degree of modification as result of the rfGD treatment. Protein and control solutions were used to incubate with the characterized SBB and, following this, MG63 osteoblast-like osteosarcoma cells were seeded over the surfaces. Cell adhesion and proliferation onto SCA was found to be enhanced for non-treated surfaces and on SCA+10%HA no alteration was brought up by the plasma modification. Onto SCA surfaces, BSA, FN and VN single solutions improved cell adhesion, and this same effect was found upscaled for ternary systems. In addition, plasma treated SEVA-C directed an increase in both adhesion and proliferation comparing to non-treated surfaces. Even though adhesion onto treated and untreated SPCL was quite similar, plasma modification clearly promoted MG63 cells proliferation. Regarding MG63 cells morphology it was shown that onto SEVA-C surfaces the variation of cell shape was primarily defined by the protein system, while onto SPCL it was mainly affected by the plasma treatment.
Catarina M Alves; Y Yang; D L Carnes; J L Ong; V L Sylvia; D D Dean; C M Agrawal; R L Reis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-02
Journal Detail:
Title:  Biomaterials     Volume:  28     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-10-18     Completed Date:  2007-01-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  307-15     Citation Subset:  IM    
3B's Research Group--Biomaterials, Biodegradables and Biomimetics, Campus Gualtar, 4710-057 Braga, Portugal.
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MeSH Terms
Biocompatible Materials / chemistry*
Bone Substitutes / chemistry
Bone and Bones / cytology*
Cell Adhesion / drug effects,  physiology*
Microscopy, Electron, Scanning
Osteoblasts / cytology,  physiology*
Proteins / chemistry
Starch / chemistry
Water / chemistry
Reg. No./Substance:
0/Biocompatible Materials; 0/Bone Substitutes; 0/Proteins; 7732-18-5/Water; 9005-25-8/Starch

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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