| Modular optimization of multi-gene pathways for fatty acids production in E. coli. | |
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MedLine Citation:
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PMID: 23361000 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Microbial fatty acid-derived fuels have emerged as promising alternatives to petroleum-based transportation fuels. Here we report a modular engineering approach that systematically removed metabolic pathway bottlenecks and led to significant titre improvements in a multi-gene fatty acid metabolic pathway. On the basis of central pathway architecture, E. coli fatty acid biosynthesis was re-cast into three modules: the upstream acetyl coenzyme A formation module; the intermediary acetyl-CoA activation module; and the downstream fatty acid synthase module. Combinatorial optimization of transcriptional levels of these three modules led to the identification of conditions that balance the supply of acetyl-CoA and consumption of malonyl-CoA/ACP. Refining protein translation efficiency by customizing ribosome binding sites for both the upstream acetyl coenzyme A formation and fatty acid synthase modules enabled further production improvement. Fed-batch cultivation of the engineered strain resulted in a final fatty acid production of 8.6 g l(-1). The modular engineering strategies demonstrate a generalized approach to engineering cell factories for valuable metabolites production. |
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Authors:
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Peng Xu; Qin Gu; Wenya Wang; Lynn Wong; Adam G W Bower; Cynthia H Collins; Mattheos A G Koffas |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature communications Volume: 4 ISSN: 2041-1723 ISO Abbreviation: Nat Commun Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-01-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101528555 Medline TA: Nat Commun Country: England |
Other Details:
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Languages: eng Pagination: 1409 Citation Subset: IM |
Affiliation:
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1] Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, New York 12180, USA [2] Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA. |
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