Document Detail

Modified use of a commercial ELISA kit for deoxynivalenol determination in rice and corn silage.
MedLine Citation:
PMID:  23275133     Owner:  NLM     Status:  Publisher    
The analysis of deoxynivalenol (DON) in silage samples using enzyme-linked immunosorbent assay (ELISA) often leads to an overestimation. To better analyze DON in rice and corn silages using a commercially available ELISA kit, a cleanup method using a MultiSep #226 column was developed. As a result, overestimation of DON by the influence of specific cross-reaction with acetyldeoxynivalenol (AcDON) was confirmed. In samples where AcDON was not detected by liquid chromatography with mass spectrometry (LC-MS), no samples showed a significant difference (P < 0.05) in DON amounts between ELISA with cleanup and LC-MS analysis. For the recovery study, blank silage was spiked with 0.5 or 1.0 mg/kg DON. The mean recoveries of DON determined by ELISA with cleanup and LC-MS analysis were 112 and 96 %, respectively, and the relative standard deviation for the repeatability (RSD(r)) were 8.2 and 9.8 %, respectively. No samples showed a significant difference (P < 0.05) in DON concentration determined by either ELISA or LC-MS analysis. A collaborative study to validate this rapid method was carried out using four samples, two rice and two corn silage, by 10 participating laboratories. Each sample was analyzed using blind duplicates. The mean values of DON detected were 1.5-2.3 mg/kg, RSD(r) and the relative standard deviation for the reproducibility (RSD(R)) were 4.1-12.7 and 7.6-23.4 %, respectively, and the HorRat values were 0.5-1.6. Therefore, the overestimation of DON by the influence of nonspecific cross-reaction with sample matrix was reduced by the cleanup method using a MultiSep #226 column, and analysis of DON in silage was improved. This use of this method for estimation of DON contamination in silage allows rapid detection at the place of use that is likely to result in improved animal health.
Hisaaki Hiraoka; Katsumi Yamamoto; Yukiko Mori; Naoki Asao; Rie Fukunaka; Kenzaburo Deguchi; Kenzi Iida; Shigeru Miyazaki; Tetsuhisa Goto
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-30
Journal Detail:
Title:  Mycotoxin research     Volume:  -     ISSN:  1867-1632     ISO Abbreviation:  Mycotoxin Res     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807334     Medline TA:  Mycotoxin Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Kobe Regional Center, Food and Agricultural Materials Inspection Center, 1-3-7 Minatojima-minami-machi, Chuo-ku, Kobe, 650-0047, Japan.
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