Document Detail

Modified liquid junction interface for nonaqueous capillary electrophoresis-mass spectrometry.
MedLine Citation:
PMID:  11001230     Owner:  NLM     Status:  MEDLINE    
Electrospray ionization (ESI) is the most widely used ionization method in on-line coupling of capillary electrophoresis-mass spectrometry (CE-MS). The conventional coaxial sheath flow electrospray interface is currently being replaced by the more sensitive nanoelectrospray technique. The usual limitation of nanoelectrospray CE-MS interface has been its short lifetime caused by deterioration of the metal coating on the CE capillary terminus. This article describes an easy way to construct a more durable and sensitive nanospray interface for nonaqueous CE-MS. In this approach a very thin glass spray capillary (ca. 30 microm outer diameter) is partly inserted inside the CE capillary, the junction being surrounded by the electrolyte medium, which is in contact with the platinum electrode. The interface was tested with five pharmaceuticals: methadone, pentazocine, levorphanol, dihydrocodeine, and morphine. Detection limits ranged from 12 to 540 fmol. Separation efficiency and reproducibility were also studied. The CE current was found to be stable and the migration times were highly reproducible. All the CE separations were carried out in a nonaqueous background electrolyte solution.
M Jussila; K Sinervo; S P Porras; M L Riekkola
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  21     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-18     Completed Date:  2001-01-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  3311-7     Citation Subset:  IM    
Department of Chemistry, University of Helsinki, Finland.
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MeSH Terms
Codeine / analogs & derivatives,  analysis,  isolation & purification
Electrophoresis, Capillary / instrumentation*,  methods
Equipment Design
Indicators and Reagents
Levorphanol / analysis,  isolation & purification
Methadone / analysis,  isolation & purification
Morphine / analysis,  isolation & purification
Pentazocine / analysis,  isolation & purification
Pharmaceutical Preparations / analysis*,  isolation & purification
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / instrumentation*,  methods
Reg. No./Substance:
0/Indicators and Reagents; 0/Pharmaceutical Preparations; 125-28-0/dihydrocodeine; 359-83-1/Pentazocine; 57-27-2/Morphine; 76-57-3/Codeine; 76-99-3/Methadone; 77-07-6/Levorphanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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