| Modified liquid junction interface for nonaqueous capillary electrophoresis-mass spectrometry. | |
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MedLine Citation:
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PMID: 11001230 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrospray ionization (ESI) is the most widely used ionization method in on-line coupling of capillary electrophoresis-mass spectrometry (CE-MS). The conventional coaxial sheath flow electrospray interface is currently being replaced by the more sensitive nanoelectrospray technique. The usual limitation of nanoelectrospray CE-MS interface has been its short lifetime caused by deterioration of the metal coating on the CE capillary terminus. This article describes an easy way to construct a more durable and sensitive nanospray interface for nonaqueous CE-MS. In this approach a very thin glass spray capillary (ca. 30 microm outer diameter) is partly inserted inside the CE capillary, the junction being surrounded by the electrolyte medium, which is in contact with the platinum electrode. The interface was tested with five pharmaceuticals: methadone, pentazocine, levorphanol, dihydrocodeine, and morphine. Detection limits ranged from 12 to 540 fmol. Separation efficiency and reproducibility were also studied. The CE current was found to be stable and the migration times were highly reproducible. All the CE separations were carried out in a nonaqueous background electrolyte solution. |
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Authors:
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M Jussila; K Sinervo; S P Porras; M L Riekkola |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Electrophoresis Volume: 21 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2001-01-18 Completed Date: 2001-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: GERMANY |
Other Details:
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Languages: eng Pagination: 3311-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Helsinki, Finland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Codeine
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analogs & derivatives,
analysis,
isolation & purification Electrophoresis, Capillary / instrumentation*, methods Equipment Design Indicators and Reagents Levorphanol / analysis, isolation & purification Methadone / analysis, isolation & purification Morphine / analysis, isolation & purification Pentazocine / analysis, isolation & purification Pharmaceutical Preparations / analysis*, isolation & purification Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Pharmaceutical Preparations; 125-28-0/dihydrocodeine; 359-83-1/Pentazocine; 57-27-2/Morphine; 76-57-3/Codeine; 76-99-3/Methadone; 77-07-6/Levorphanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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