Document Detail


Modified giemsa-11 staining protocol for chromosomes of human and hybrid cells.
MedLine Citation:
PMID:  10226656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Giemsa-11 or G-11 is a specialized staining technique utilized to (1) differentiate heterochromatic regions of human chromosomes, (2) identify the presence of human chromosomes in human-rodent hybrid cells, and (3) identify human-rodent translocation products in hybrid cells. Earlier procedures, though useful, are problematic and may fail to yield results due to inadequate differentiation between light and dark staining regions. The improved protocol presented here is easy, reliable, and applicable in both clinical and research situations. A discussion of the biology of the staining process is also given.
Authors:
C K Stein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Somatic cell and molecular genetics     Volume:  24     ISSN:  0740-7750     ISO Abbreviation:  Somat. Cell Mol. Genet.     Publication Date:  1998 May 
Date Detail:
Created Date:  1999-05-13     Completed Date:  1999-05-13     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8403568     Medline TA:  Somat Cell Mol Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-5     Citation Subset:  IM    
Affiliation:
Department of Pathology, State University of New York Health Science Center, Syracuse, New York 13210, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Azure Stains*
Chromosomes / chemistry,  genetics
Chromosomes, Human / chemistry*,  genetics
Cricetinae
Cytogenetics / methods
Humans
Hybrid Cells / chemistry*,  metabolism
Metaphase
Staining and Labeling / methods*
Chemical
Reg. No./Substance:
0/Azure Stains

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