Document Detail


Modified and derived ethanol injection toward liposomes: development of the process.
MedLine Citation:
PMID:  23020802     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A modified and derived ethanol injection (MDEI) process was developed to produce liposomes. The aim of the present study was to more efficiently control the vesicle diameter than with the conventional ethanol injection method. A hot ethanolic solution of lipids (60°C) was injected into a hot aqueous buffer (70°C). Then, ethanol was removed by rotary evaporation under reduced pressure. The size of the liposomes could be controlled by the ratio of ethanol to hydroalcoholic solution before evaporation. The concentration of lipids, the charge of lipids, and the type of aqueous phase had little effect on the vesicle diameter when the process involved a ratio of 33% (v/v) ethanol. In addition, it was possible to obtain lipid concentrations 10- to 30-fold higher that the conventional ethanol injection method. The encapsulation of a hydrophilic compound was feasible with this MDEI process. The observation by cryogenic transmission electron microscopy revealed that these liposomes were predominantly unilamellar at a ratio as high as 33 or 50% (v/v) ethanol. Thus, the results showed that MDEI is an appropriate alternative for the manufacture of liposomes with respect to the ethanol injection process.
Authors:
Philippe Gentine; Line Bourel-Bonnet; Benoît Frisch
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  Journal of liposome research     Volume:  -     ISSN:  1532-2394     ISO Abbreviation:  J Liposome Res     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001952     Medline TA:  J Liposome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratoire de Conception et Application de Molécules Bioactives, Equipe de BioVectorologie, UMR 7199 CNRS/Université de Strasbourg, Faculté de Pharmacie , Illkirch , France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  On testing an unspecified function through a linear mixed effects model with multiple variance compo...
Next Document:  TWO NEW SPECIES OF RHABDIAS (NEMATODA: RHABDITIDA: RHABDIASIDAE) IN ANURAN HOSTS FROM DEHRADUN (UTTA...