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Modified binding of proteins from calcitonin-negative tumor cells to the neuroendocrine-specific CANNTG motif of the calcitonin gene.
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MedLine Citation:
PMID:  8265349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcription of the calcitonin (CT) gene in the medullary thyroid carcinoma (MTC) cell line TT is modulated by a neuroendocrine-specific enhancer fragment (nucleotides -965 to -905) containing two CANNTG motifs (E2 and E3) and an ETs-like response element. To determine the cell-specific component of this fragment, oligonucleotides containing the individual elements were inserted in front of a minimal CT promoter and tested for reporter protein production in CT-positive (TT) and -negative (RO-D81 and HeLa) cells. In TT cells, using two copies of E2 or four copies of Ets increased minimal promoter activity a 20-40 fold. Using two copies of E3 had no effect on minimal promoter activity. In CT-negative MTC cells (RO-D81), the Ets response element was active but the two copies of E2 were not. Similar results were obtained with the non-neuroendocrine cell-line HeLa. I therefore concluded that E2 was the cell-type-specific component of the enhancer. An E2-specific binding protein was detected in both MTC cell lines but not in HeLa. This protein had different mobility and DNA-binding specificity in CT-positive TT cells and CT-negative RO-D81 cells. In conclusion, the CAGCTG motif of E2 modulated the cell-specific transcription of the CT gene, and its inactivation in CT-negative MTC cells correlated with modifications in its binding proteins.
Authors:
S Peleg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  21     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-01-21     Completed Date:  1994-01-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  5360-5     Citation Subset:  IM    
Affiliation:
Department of Medical Specialties, University of Texas M.D. Anderson Cancer Center, Houston 77030.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Calcitonin / genetics*
Carcinoma, Medullary / genetics*
Consensus Sequence
DNA-Binding Proteins / metabolism*
Enhancer Elements, Genetic*
Gene Expression Regulation, Neoplastic*
Hela Cells
Humans
Molecular Sequence Data
Oligodeoxyribonucleotides / chemistry
RNA, Messenger / genetics
Thyroid Neoplasms / genetics*
Transcription Factors / metabolism
Grant Support
ID/Acronym/Agency:
2P30CA16672-018/CA/NCI NIH HHS; R01-DK38146/DK/NIDDK NIH HHS; RR-05425/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 0/Transcription Factors; 9007-12-9/Calcitonin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 11 Year: 1993
Volume: 21 Issue: 23
First Page: 5360 Last Page: 5365
ID: 310571
PubMed Id: 8265349

Modified binding of proteins from calcitonin-negative tumor cells to the neuroendocrine-specific CANNTG motif of the calcitonin gene.
S Peleg
Department of Medical Specialties, University of Texas M.D. Anderson Cancer Center, Houston 77030.



Article Categories:
  • Research Article


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