| Modified arabinoxylan rice bran (MGN3/Biobran) enhances intracellular killing of microbes by human phagocytic cells in vitro. | |
| | |
MedLine Citation:
|
PMID: 18336734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phagocytic cells, comprised of neutrophils and monocytes/macrophages, play a key role in the innate immune response to infection. Our earlier study demonstrated that arabinoxylan rice bran (MGN-3/Biobran) activates murine peritoneal macrophage and macrophage cell lines. In this study, we investigated whether MGN-3 can upregulate the phagocytic activity of human phagocytes in peripheral blood to phagocytize Escherichia coli (E. coli), trigger the oxidative burst and produce cytokines. Phagocytic cells were pre-labeled with dichlorofluorescin diacetate dye and were incubated with phycoerythrin-labeled E. coli in the presence or absence of MGN-3. Phagocytosis and oxidative burst were assessed by flow cytometry. Results showed that treatment with MGN-3 enhanced the phagocytosis of E. coli by neutrophils and monocytes. This was associated with an increased oxidative burst. In addition, it caused a significant induction of cytokines (TNF-alpha, IL-6, IL-8 and IL-10); the effect was detected at 1 microg/ml and increased in a dose-dependent manner (P <or= 0.01). Notably, MGN-3 alone had no effect on the growth of 31 strains of bacteria suggesting that MGN-3 modulates phagocytic cellular function. These findings may have applications in the treatment of infections in the elderly and in immunocompromised patients. |
| | |
Authors:
|
M Ghoneum; M Matsuura; S Gollapudi |
Related Documents
:
|
21782444 - Nuclear factor-κb and apoptosis in patients with intracerebral hemorrhage. 7420024 - Effects of soluble immune complexes on fc receptor- and c3b receptor-mediated phagocyto... 21835894 - Transplantation of β-endorphin neurons into the hypothalamus promotes immune function ... 20973804 - Alternatively activated macrophages and impaired phagocytosis of s. aureus in chronic ... 6192094 - Inhibition of passive localized experimental allergic encephalomyelitis by interferon. 17689554 - Murine ctll-2 cells respond to mil12: prospects for developing an alternative bioassay ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of immunopathology and pharmacology Volume: 21 ISSN: 0394-6320 ISO Abbreviation: Int J Immunopathol Pharmacol Publication Date: 2008 Jan-Mar |
Date Detail:
|
Created Date: 2008-03-13 Completed Date: 2008-06-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8911335 Medline TA: Int J Immunopathol Pharmacol Country: Italy |
Other Details:
|
Languages: eng Pagination: 87-95 Citation Subset: IM |
Affiliation:
|
Department of Otolaryngology, Drew University of Medicine and Science, Los Angeles, CA 90059, USA. mghoneum@ucla.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteria
/
drug effects Cell Line Cytokines / biosynthesis Humans Phagocytes / drug effects*, immunology Phagocytosis / drug effects* Respiratory Burst / drug effects Xylans / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Xylans; 0/polysaccharide MGN3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Omeprazole induces apoptosis in normal human polymorphonuclear leucocytes.
Next Document: Expression of CD38 in human neuroblastoma SH-SY5Y cells.