Document Detail


Modified C-reactive protein interacts with platelet glycoprotein Ibα.
MedLine Citation:
PMID:  21602602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Herein, we investigated the possible mechanisms by which recombinant modified CRP(m(r)CRP) modulates blood platelet function. Modified CRP could activate blood platelets and stimulate their adhesion and aggregation in the absence of any other physiological stimuli. Preincubation of isolated blood platelets with m(r)CRP at a concentration as low as 2 μg/ml resulted in significant platelet degranulation (fraction of CD62-positive platelets increased 2-fold, p < 0.0002), and at concentrations of 20 μg/ml and 100 μg/ml, increased exposure of the platelet procoagulant surface was observed (expression of annexin V-positive platelets increased to 5.7 ± 1.0% and 10.4 ± 2.2%, respectively, p < 0.03, vs. 2.9 ± 0.2% in control). Furthermore, m(r)CRP (100 μg/ml) strongly augmented spontaneous and ADP-induced fibrinogen binding to platelets (p < 0.05), platelet adhesion to fibrinogen and platelet aggregation. Using the Biacore™ surface plasmon resonance technique and glycoprotein Ibα (GPIbα) immobilized on the sensor surface, we demonstrated direct binding between platelet GPIbα and m(r)CRP. Binding of m(r)CRP to GPIbα and C1q was also observed by ELISA, irrespective of the immobilized ligand. These outcomes strongly support a role of the GPIb-IX-V complex in the interactions of m(r)CRP with blood platelets.
Authors:
Magdalena Boncler; Joanna Rywaniak; Jacek Szymański; Lawrence A Potempa; Błażej Rychlik; Cezary Watała
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pharmacological reports : PR     Volume:  63     ISSN:  1734-1140     ISO Abbreviation:  Pharmacol Rep     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-23     Completed Date:  2011-09-16     Revised Date:  2011-11-28    
Medline Journal Info:
Nlm Unique ID:  101234999     Medline TA:  Pharmacol Rep     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  464-75     Citation Subset:  IM    
Affiliation:
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Żeromskiego 113, PL 90-549 Łódź, Poland. mboncler@csk.umed.lodz.pl
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MeSH Terms
Descriptor/Qualifier:
Adult
Annexin A5 / metabolism
Blood Platelets / metabolism*
C-Reactive Protein / administration & dosage,  metabolism*
Enzyme-Linked Immunosorbent Assay
Female
Fibrinogen / metabolism
Humans
Male
Platelet Adhesiveness
Platelet Aggregation
Platelet Glycoprotein GPIb-IX Complex / metabolism*
Protein Binding
Recombinant Proteins
Surface Plasmon Resonance
Young Adult
Chemical
Reg. No./Substance:
0/Annexin A5; 0/Platelet Glycoprotein GPIb-IX Complex; 0/Recombinant Proteins; 9001-32-5/Fibrinogen; 9007-41-4/C-Reactive Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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