| Modifications of a calcium phosphate cement with biomolecules--influence on nanostructure, material, and biological properties. | |
| | |
MedLine Citation:
|
PMID: 20845493 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Calcium phosphate cements (CPC), forming hydroxyapatite during the setting reaction, are characterized by good biocompatibility and osteoconductivity, however, their remodeling into native bone tissue is slow. One strategy to improve remodeling and bone regeneration is the directed modification of their nanostructure. In this study, a CPC was set in the presence of cocarboxylase, glucuronic acid, tartaric acid, α-glucose-1-phosphate, L-arginine, L-aspartic acid, and L-lysine, respectively, with the aim to influence formation and growth of hydroxyapatite crystals through the functional groups of these biomolecules. Except for glucuronic acid, all these modifications resulted in the formation of smaller and more agglomerated hydroxyapatite particles which had a positive impact on the biological performance indicated by first experiments with the human osteoblast cell line hFOB 1.19. Moreover, adhesion, proliferation, and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSC) as well as binding of the growth factors BMP-2 and VEGF was investigated on CPC modified with cocarboxylase, arginine, and aspartic acid. Initial adhesion of hBMSC was improved on these three modifications and proliferation was enhanced on CPC modified with cocarboxylase and arginine whereas osteogenic differentiation remained unaffected. Modification of the CPC with arginine and aspartic acid, but not with cocarboxylase, led to a higher BMP-2 binding. |
| | |
Authors:
|
Corina Vater; Anja Lode; Anne Bernhardt; Antje Reinstorf; Berthold Nies; Michael Gelinsky |
Related Documents
:
|
17095153 - Specific growth rate of sulfate reducing bacteria in the presence of manganese and cadm... 21956573 - Biosensors for d: -amino acid detection. 19171303 - Engineering a function into a glycosyltransferase. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biomedical materials research. Part A Volume: 95 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-10-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
|
Languages: eng Pagination: 912-23 Citation Subset: IM |
Copyright Information:
|
© 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010. |
Affiliation:
|
Max Bergmann Center of Biomaterials and Institute for Materials Science, Technische Universität Dresden, Budapester Str 27, D-01069 Dresden, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Latex use as an occlusive membrane for guided bone regeneration.
Next Document: Cytoskeletal dynamics in response to tensile loading of mammalian axons.