| Modifications of RNase protection assay for neuroscience applications. | |
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MedLine Citation:
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PMID: 9821645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This report describes how certain modifications of the Ribonuclease (RNase) protection assay may increase its efficiency by decreasing the time and cost of the procedure without compromising reliability. We show that, under the experimental conditions tested, the RNA samples can be precipitated by a solution of Tri Reagent in ethanol immediately following the RNase digestion step. Drying the samples under vacuum before dissolving them in the gel loading buffer improves the consistency of the assay as compared to air drying. Although these modifications are applicable to the RNase protection assay in general, we present an example that used a multiprobe set we developed and have used effectively in the analysis of cytokine mRNA regulation in the brain. |
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Authors:
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S E Ilyin; D Gayle; C R Plata-Salamán |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 84 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1999-01-26 Completed Date: 1999-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 139-41 Citation Subset: IM |
Affiliation:
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Division of Molecular Biology, School of Life and Health Sciences, University of Delaware, Newark 19716-2590, USA. 78742@udel.eud |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Cytokines / genetics Gene Expression Regulation Indicators and Reagents Liver / metabolism Neurosciences / methods* RNA / analysis*, isolation & purification RNA, Messenger / analysis, genetics Rats Reproducibility of Results Ribonucleases / metabolism* Spleen / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Indicators and Reagents; 0/RNA, Messenger; 63231-63-0/RNA; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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