Document Detail

Modification of the volatile compound profile of cheese, by a Lactococcus lactis strain expressing a mutant oligopeptide binding protein.
MedLine Citation:
PMID:  18226303     Owner:  NLM     Status:  MEDLINE    
Lactococcus lactis strain AMP2I expresses OppA(D471R), a mutant oligopeptide binding OppA protein in which the aspartyl residue at position 471 was replaced by arginine. As a consequence of a different peptide transport in this strain, experimental Hispánico cheese made with Lc. lactis AMP2I had a higher content of total free amino acids than control cheese made with Lc. lactis AMP1I, an isogenic strain expressing wild-type OppA (Picon et al. 2005, 2007). In this work higher levels of diketones, hydroxy-ketones and, to a lesser extent, branched chain aldehydes were recorded for experimental cheese compared with control cheese. These differences levelled off as ripening proceeded. Strong correlations support the hypothesis that the increased levels of these volatile compounds in cheese made with Lc. lactis AMP2I are linked to higher concentrations of free amino acids threonine, valine and leucine.
Antonia Picon; Estrella Fernández-García; Pilar Gaya; Manuel Nuñez
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Publication Detail:
Type:  Journal Article     Date:  2008-01-29
Journal Detail:
Title:  The Journal of dairy research     Volume:  75     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-10-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  30-6     Citation Subset:  IM    
Departamento de Tecnología de Alimentos, SGIT-INIA, Carretera de La Coruña Km 7.5, 28040 Madrid Spain.
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MeSH Terms
Bacterial Proteins / genetics*,  metabolism*
Carrier Proteins / genetics*,  metabolism*
Cheese / analysis*,  microbiology
Food Microbiology
Hydrogen-Ion Concentration
Lactococcus lactis / genetics,  metabolism*
Lipoproteins / genetics*,  metabolism*
Organic Chemicals / chemistry
Reg. No./Substance:
0/Bacterial Proteins; 0/Carrier Proteins; 0/Lipoproteins; 0/Oligopeptides; 0/Organic Chemicals; 0/oligopeptide-binding protein, bacteria

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